C. jejuni and S. typhimurium are the leading causes of bacterial food contamination in chicken carcasses. Contamination is particularly associated with the slaughtering process. This study isolated C. jejuni and S. typhimurim from fifty chicken carcass samples, all of which were acquired from different companies in Riyadh, Saudi Arabia. The identification of C. jejuni was performed phenotypically by using a hippurate test and genetically using a polymerase chain reaction with primers for 16S rRNA and hippurate hydrolase (hipO gene). For the dentification of S. typhimurim, a serological Widal test was carried out using serum antiS. typhimurium antibodies. Samonella genetically detected using invA gene primers. The positive isolates for C. jejuni showed a specific molecular size of 1448 bp for 16S rRNA and 1148 bp for hipO genes. However, the positive isolates of the invA gene exhibited a specific molecular size at 244 bp using polymerase chain reaction. Comparing sequencing was performed with respect to the invA gene and the BLAST nucleotide isolates that were identified as Salmonella enterica subsp. enterica serovar typhimurium strain ST45, thereby producing a similarity of 100%. The testing identified C. jejuni for hippuricase, GenBank: Z36940.1. Many isolates of Salmonella spp. that contained the invA gene were not necessarily identified as S. typhimurim, the limiting factor for the Widal test used antiS. typhimurum antibodies. The multidrug resistance of C. jejuni isolates in chickens was compared with the standard C. jejuni strain ATCC 22931. Similarly, S. typhimurium isolates were compared with the standard S. typhimurium strain ATCC 14028.

中文翻译：

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使用 PCR 检测毒力因子 hipO 和 invA 基因（沙特阿拉伯）检测鸡中的空肠弯曲杆菌和鼠伤寒沙门氏菌。

C. jejuni 和 S. typhimurium 是鸡尸体中细菌性食物污染的主要原因。污染尤其与屠宰过程有关。本研究从 50 只鸡胴体样本中分离出空肠弯曲菌和鼠伤寒沙门氏菌，所有这些样本均购自沙特阿拉伯利雅得的不同公司。空肠弯曲杆菌的鉴定通过使用马尿酸测试和遗传使用聚合酶链反应与 16S rRNA 和马尿酸水解酶（hipO 基因）的引物进行表型鉴定。为了鉴定鼠伤寒沙门氏菌，使用血清 antiS 进行了血清学 Widal 试验。鼠伤寒抗体。使用 invA 基因引物对沙门氏菌进行基因检测。空肠弯曲杆菌的阳性分离株显示出 16S rRNA 的特定分子大小为 1448 bp，hipO 基因的特定分子大小为 1148 bp。然而，使用聚合酶链反应，invA 基因的阳性分离物在 244 bp 处表现出特定的分子大小。对 invA 基因和鉴定为肠沙门氏菌亚种的 BLAST 核苷酸分离物进行比较测序。enterica serovar typhimurium 菌株 ST45，从而产生 100% 的相似性。该测试确定了用于马尿酸酶的空肠弯曲菌，GenBank：Z36940.1。许多沙门氏菌分离株。含有 invA 基因的病毒不一定被鉴定为 S. typhimurim，Widal 测试的限制因素是使用 antiS。鼠伤寒抗体。将鸡中空肠杆菌分离株的多药耐药性与标准空肠弯曲杆菌菌株 ATCC 22931 进行比较。类似地，将鼠伤寒沙门氏菌分离株与标准鼠伤寒沙门氏菌菌株 ATCC 14028 进行比较。