In recent years green nanotechnology gained significant importance to synthesize nanoparticles due to their cost effectiveness and biosafety. In the current study, silver nanoparticles were synthesized by using extract of Spirogyra hyalina as a capping and reducing agent. The synthesized nanoparticles were characterized by UV–Visible spectroscopy, Fourier transform infrared spectroscopy, Scanning electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffractive analysis. Silver nanoparticles give a characteristic Surface Plasmon Resonance peak of 451 nm at 2.21 a.u (arbitrary unit). SEM micrograph revealed the spherical morphology and average grain size of 52.7 nm. Furthermore, antibacterial, antifungal, insecticidal, antioxidant and membrane damage activities were determined. The maximum antibacterial and antifungal activity was observed for Pseudomonas aeruginosa (18 ± 1.2 mm) and Fusarium solani (14.3 ± 0.6 mm), respectively. In membrane damage assay, Pseudomonas aeruginosa absorbed A₂₆₀ wavelength and gave maximum peak values of 0.286, 0.434 and 0.629 at 25, 35 and 45 µg/mL of silver nanoparticles. The membrane damage assay confirmed that nanoparticles are involved in bacterial cell membrane damage. At 500 ppm silver nanoparticles showed 30% mortality against Tribolium castaneum (a common grain pest). The silver nanoparticles also showed potent antioxidant activity and successfully scavenged the DPPH free radicals upto 53.43 ± 0.17, 43.26 ± 0.97, 31.39 ± 0.33, 24.62 ± 0.85, and 14.13 ± 0.12% at a concentration of 400, 200, 100, 50, and 25 µg/mL of nanoparticles, respectively. It is concluded that silver nanoparticles can easily be synthesized by using green algae Spirogyra hyalina as a capping and reducing agent. Silver nanoparticles showed potent biomedical activities and thus can be used for therapeutic applications invitro and invivo.

近年来，由于其成本效益和生物安全性，绿色纳米技术在合成纳米颗粒方面变得非常重要。在目前的研究中，使用透明水绵提取物合成银纳米粒子作为封端剂和还原剂。通过紫外-可见光谱、傅里叶变换红外光谱、扫描电子显微镜、能量色散 X 射线光谱和 X 射线衍射分析对合成的纳米颗粒进行了表征。银纳米粒子在 2.21 au（任意单位）处产生 451 nm 的特征表面等离子体共振峰。SEM显微照片显示球形形态和52.7nm的平均晶粒尺寸。此外，还测定了抗菌、抗真菌、杀虫、抗氧化和膜损伤活性。分别观察到铜绿假单胞菌(18 ± 1.2 mm) 和茄病镰刀菌(14.3 ± 0.6 mm)的最大抗菌和抗真菌活性。在膜损伤测定中，铜绿假单胞菌在 25、35 和 45 µg/mL 的银纳米粒子下吸收 A ₂₆₀波长并给出 0.286、0.434 和 0.629 的最大峰值。膜损伤测定证实纳米颗粒与细菌细胞膜损伤有关。在 500 ppm 时，银纳米粒子对赤刺栗（一种常见的谷物害虫）的死亡率为 30% 。银纳米粒子还显示出有效的抗氧化活性，并在浓度为 400、200、100、50 和分别为 25 µg/mL 的纳米颗粒。得出的结论是，使用绿藻Spirogyra hyalina可以很容易地合成银纳米粒子作为封端剂和还原剂。银纳米粒子显示出强大的生物医学活性，因此可用于体外和体内治疗应用。