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Genome-wide identification of FRK genes in Populus trichocarpa and their expression under different nitrogen treatments
Physiology and Molecular Biology of Plants ( IF 3.5 ) Pub Date : 2021-09-13 , DOI: 10.1007/s12298-021-01055-6
Zhuang Zuo 1, 2, 3 , Xue Sun 1, 2 , Lina Cao 1, 2 , Shuang Zhang 4, 5 , Jiajie Yu 1, 2 , Xiuyue Xu 1, 2 , Zhiru Xu 1, 4, 5 , Guanjun Liu 1, 2 , Chunpu Qu 1, 2, 6
Affiliation  

Fructokinase (FRK) is the main fructose phosphorylase and plays an important role in catalyzing the irreversible reaction of free fructose phosphorylation. In order to study the regulatory effect of different forms and concentrations of nitrogen on PtFRK genes in Populus trichocarpa, seven genes encoding the hypothetical FRK proteins were identified in Populus trichocarpa genome by bioinformatics method. Phylogenetic analysis revealed that PtFRK family genes can be divided into two subgroups: SI (PtFRK 1, 3, 4, 6) and SII (PtFRK 2, 5, 7). The tissue-specific expression data obtained from PopGenIE indicate that PtFRK2, 3, 4 and 5 are expressed highly in the stem. Quantitative real-time RT-PCR illustrate that PtFRK1-7 showed different expression patterns in different tissues under different concentrations and morphological nitrogen application. Under high nitrate treatment, the expression levels of PtFRK1, 2, 3 and 6 in stem increased significantly, while under low nitrate treatment, only the expression of PtFRK1, 4 in the upper stem and the expression of PtFRK3, 5 in the lower stem increased significantly. In contrast, ammonium tends to inhibit the expression of PtFRKs in lower stems, the expression levels of PtFRK2, 3, 4 and 5 are significantly reduced under ammonium treatment. However, high ammonium had significant effects on PtFRK6 in the apical bud and upper leaves, which were 6 and 8 times of the control, respectively. These results laid the foundation for the study of the PtFRK gene family of poplar and provided a theoretical basis for the molecular mechanism of nitrogen regulating cell wall development.



中文翻译:

毛果杨FRK基因的全基因组鉴定及其在不同氮处理下的表达

果糖激酶(FRK)是主要的果糖磷酸化酶,在催化游离果糖磷酸化的不可逆反应中起重要作用。为了研究不同形式和浓度的氮对毛果杨PtFRK基因的调控作用,采用生物信息学方法在毛果杨基因组中鉴定出7个编码假想FRK蛋白的基因。系统发育分析表明,PtFRK家族基因可分为两个亚群:SI ( PtFRK 1, 3, 4, 6 ) 和 SII ( PtFRK 2, 5, 7 )。从 PopGenIE 获得的组织特异性表达数据表明PtFRK2、3、45在茎中高度表达。定量实时 RT-PCR 表明PtFRK1-7在不同浓度和形态施氮条件下在不同组织中表现出不同的表达模式。高硝酸处理下,茎中PtFRK1、2、3、6的表达量显增加,而低硝酸处理下,仅上茎PtFRK1、4和茎PtFRK3、5表达增加显著地。相比之下,铵往往会抑制下茎中PtFRKs的表达, PtFRK2、3、45的表达水平在铵处理下显着降低。但高铵对顶芽和上部叶片PtFRK6的影响显着,分别是对照的6倍和8倍。这些结果为杨树PtFRK基因家族的研究奠定了基础,也为氮调控细胞壁发育的分子机制提供了理论依据。

更新日期:2021-09-14
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