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Prostaglandin A2 induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda
Archives of Insect Biochemistry and Physiology ( IF 2.2 ) Pub Date : 2021-09-13 , DOI: 10.1002/arch.21844
Yong Wang 1 , Cynthia L Goodman 2 , Joseph Ringbauer 2 , Yaofa Li 3 , David Stanley 2
Affiliation  

Animals maintain homeostasis of cell numbers, constantly creating new cells and eliminating others. Programmed cell death, apoptosis, is a mechanism of cell elimination and it acts in many aspects of animal biology. Drawing on the biomedical background, several signals launch the apoptosis mechanisms, including prostaglandins (PGs). Based on this information, we posed the hypothesis that PGs similarly induce apoptosis in insect cell lines. We used three Spodoptera frugiperda cell lines, including two newly established, BCIRL-SfNS-0518B-YL derived from the central nervous system and BCIRL-Sf4FB-0614-SGS derived from fat body, and the commercially available Sf9 cells. Using a kinetic apoptosis kit, we found treating SfNS cells for 18 h with 15 or 20 μM PGA2 led to decreases in cell numbers, coupled with increased numbers of apoptotic and dead cells. Similar exposures to 10 μM PGA2 (24 h) led to substantial increases in apoptotic cells, confirmed by a terminal deoxynucleotidyl transferase dUTP nick end labeling assay on a flow cytometer. The influence of PGA2 treatments increased with dosage, as we recorded about 20% apoptosis at 24 h post-PGA2 treatments (10 μM) and about 34% apoptosis at 24 h post-30 μM treatments. PGA2 treatments led to 10- to 30-fold increases in messenger RNAs (mRNAs) encoding apoptosis-specific caspases-1, −2, −3, and −5 at 12 h and 40- to 60-fold increases in mRNAs encoding caspases-1 and −2, 10-fold increases for caspases-3 and −5 at 24 h. These findings strongly support our hypothesis that PGs induce apoptosis in an insect cell line and confirm an additional PG action in insect biology.

中文翻译:

前列腺素 A2 诱导草地贪夜蛾 Spodoptera frugiperda 三种细胞系的细胞凋亡

动物维持细胞数量的稳态,不断创造新细胞并消灭其他细胞。程序性细胞死亡、细胞凋亡是一种细胞消除机制,它在动物生物学的许多方面发挥作用。借鉴生物医学背景,一些信号启动了细胞凋亡机制,包括前列腺素 (PG)。基于这些信息,我们提出了 PGs 类似地诱导昆虫细胞系凋亡的假设。我们使用了三种草地贪夜蛾细胞系,包括两种新建立的源自中枢神经系统的 BCIRL-SfNS-0518B-YL 和源自脂肪体的 BCIRL-Sf4FB-0614-SGS,以及市售的 Sf9 细胞。使用动力学细胞凋亡试剂盒,我们发现用 15 或 20 μM PGA 2处理 SfNS 细胞 18 小时导致细胞数量减少,伴随着凋亡和死细胞数量的增加。类似地暴露于 10 μM PGA 2(24 小时)会导致凋亡细胞显着增加,这通过流式细胞仪上的末端脱氧核苷酸转移酶 dUTP 缺口末端标记测定得到证实。PGA 2处理的影响随剂量增加而增加,因为我们记录到在 PGA 2处理 (10 μM) 后 24 小时约有 20% 的细胞凋亡,在 30 μM 处理后 24 小时约有 34% 的细胞凋亡。PGA 2处理导致编码细胞凋亡特异性 caspase-1、-2、-3 和 -5 的信使 RNA (mRNA) 在 12 小时增加 10 到 30 倍,编码 caspase-1 的 mRNA 增加 40 到 60 倍和 -2,caspases-3 和 -5 在 24 小时增加 10 倍。这些发现有力地支持了我们的假设,即 PG 在昆虫细胞系中诱导细胞凋亡,并证实了 PG 在昆虫生物学中的额外作用。
更新日期:2021-10-12
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