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Triclosan elicited biochemical and transcriptomic alterations in Labeo rohita larvae
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2021-09-14 , DOI: 10.1016/j.etap.2021.103748
Sunil Sharma 1 , Owias Iqbal Dar 1 , Kirpal Singh 1 , Arvinder Kaur 1 , Caterina Faggio 2
Affiliation  

In the current study, Triclosan (TCS, a commonly used antimicrobial agent) induced alterations in biochemical parameters and gene expression were recorded in the larvae of Labeo rohita after 96 h exposure and 10 days recovery period to find out health status biomarkers. 96 h exposure to 0.06, 0.067 and 0.097 mg/L TCS significantly declined the levels of glucose, triglycerides, urea and uric acid and activity of alkaline phosphatase (ALP), glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT). There was a non-significant decline in the levels of cholesterol and total protein but albumin and total bilirubin showed no change. After 10 days of recovery period, trend was opposite for glucose, urea and ALP only. Decline in the expression of trypsin and pancreatic amylase and elevation in creatine kinase during exposure to TCS showed a reverse trend after recovery period. However, concentration dependent elevation of chymotrypsin persisted till the end of recovery period. Principal Component Analysis (PCA) showed association of total protein, ALP, GOT, creatine kinase and pancreatic amylase with PC1 after exposure as well as recovery period. Therefore, these can be considered as important biomolecules for identification of health status of TCS stressed fish.



中文翻译:

三氯生引起 Labeo rohita 幼虫的生化和转录组学改变

在目前的研究中,在Labeo rohita幼虫中记录了三氯生(TCS,一种常用的抗菌剂)诱导的生化参数和基因表达的改变经过 96 小时的暴露和 10 天的恢复期,以找出健康状况的生物标志物。暴露于 0.06、0.067 和 0.097 mg/L TCS 96 小时后,葡萄糖、甘油三酯、尿素和尿酸的水平以及碱性磷酸酶 (ALP)、谷氨酸草酰乙酸转氨酶 (GOT) 和谷氨酸丙酮酸转氨酶 (GPT) 的活性显着下降。胆固醇和总蛋白水平没有显着下降,但白蛋白和总胆红素没有变化。10 天恢复期后,仅葡萄糖、尿素和 ALP 的趋势相反。在恢复期后,暴露于 TCS 期间胰蛋白酶和胰淀粉酶表达的下降和肌酸激酶的升高显示出相反的趋势。然而,胰凝乳蛋白酶的浓度依赖性升高持续到恢复期结束。主成分分析 (PCA) 显示总蛋白、ALP、GOT、肌酸激酶和胰淀粉酶与暴露后和恢复期的 PC1 相关。因此,这些可以被认为是识别 TCS 应激鱼类健康状况的重要生物分子。

更新日期:2021-09-20
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