This is a feasibility study on the ability of biodegradable collagen coated with poly(L-lactide-co-ε-caprolactone) (PLC) to deliver the antimicrobial peptide, LL-37, for usage as a barrier membrane in guided bone regeneration. In this study, a nanofibrous PLC is fabricated using the electrospinning technique to establish the controlled release of the LL-37, which adsorbed onto collagen scaffold (LL/Col), in order to produce a LL/Col/PLC membrane. The in vitro released profiles of the LL-37 obtained from the LL/Col/PLC membranes are studied in phosphate buffer saline and are compared with unfabricated LL/Col membranes. According to the results, the LL/Col/PLC membrane is capable of delivering LL-37 in a sustained release in amounts that has no cytotoxicity over a period of 14 days. An assessment of the biocompatibility of the LL/Col/PLC membrane indicate that it can enhance the proliferation of mouse fibroblasts (L929) and human monocytes that are known to be a key regulator in the bone healing process. Moreover, the surface topography of the LL/Col/PLC membrane mimics the extracellular matrix by establishing cell attachment and proliferation. These findings indicate that the LL/Col/PLC membrane developed in this study can sustain the release of LL-37 in clinically relevant doses, which could then further enhance bone regeneration.

中文翻译：

---

基于胶原蛋白和 PLC 的双相纳米纤维支架在引导骨再生中用于控释 LL-37

这是可生物降解的胶原的涂有聚（L丙交酯的能力的可行性研究共- ε-己内酯) (PLC) 提供抗菌肽 LL-37，用作引导骨再生中的屏障膜。在这项研究中，使用静电纺丝技术制造了纳米纤维 PLC，以建立 LL-37 的受控释放，LL-37 吸附在胶原支架 (LL/Col) 上，以生产 LL/Col/PLC 膜。在磷酸盐缓冲盐水中研究从 LL/Col/PLC 膜获得的 LL-37 的体外释放曲线，并与未加工的 LL/Col 膜进行比较。根据结果​​，LL/Col/PLC 膜能够以在 14 天内没有细胞毒性的量持续释放 LL-37。对 LL/Col/PLC 膜生物相容性的评估表明，它可以增强小鼠成纤维细胞 (L929) 和人类单核细胞的增殖，这些细胞是骨愈合过程中的关键调节剂。此外，LL/Col/PLC 膜的表面形貌通过建立细胞附着和增殖来模拟细胞外基质。这些发现表明，本研究中开发的 LL/Col/PLC 膜可以维持临床相关剂量的 LL-37 的释放，从而进一步增强骨再生。