Two iridium (III) polypyridine complexes [Ir(ppy)₂(BIP)]PF₆ (ppy = 2-phenylpyridine, BIP = 2-biphenyl-1H-imidazo[4,5-f][1,10]phenanthroline, Ir1), [Ir(piq)₂(BIP)]PF₆ (piq = 1-phenylisoquinoline, Ir2) and their liposomes Ir1lipo and Ir2lipo were synthesized and characterized. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cytotoxic activity against several cancer cells (A549, HepG2, SGC-7901, Bel-7402, HeLa) and non-cancer cell (mouse embryonic fibroblast, NIH3T3). The results showed that Ir1lipo displays the high cytotoxicity toward SGC-7901 with IC₅₀ value of 5.8 ± 0.2 μM, while the complexes have no cytotoxicity toward A549, HepG2, Bel-7402 and HeLa cells. The cell colony demonstrated that the iridium (III) complexes-loaded liposomes can inhibit cell proliferation, induce cell cycle arrest at G0/G1 phase. Moreover, they also cause autophagy, induce a decrease of mitochondrial membrane potential and increase intracellular reactive oxygen species (ROS) content. These results suggest that the complexes encapsulated liposomes Ir1lipo and Ir2lipo inhibit the growth of SGC-7901 cells through a ROS-mediated mitochondrial dysfunction and activating the PI3K (phosphoinositide-3 kinase)/ AKT (protein kinase B) signaling pathways.

两个铱 (III) 多吡啶配合物 [Ir(ppy) ₂ (BIP)]PF ₆ (ppy = 2-苯基吡啶, BIP = 2-biphenyl-1 H-咪唑并[4,5-f][1,10]菲咯啉,合成并表征了Ir1 )、[Ir(piq) ₂ (BIP)]PF ₆ (piq = 1-苯基异喹啉、Ir2 ) 及其脂质体Ir1lipo和Ir2lipo。3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 测定用于评估对几种癌细胞（A549、HepG2、SGC-7901、Bel-7402、HeLa）和非癌细胞（小鼠胚胎成纤维细胞，NIH3T3）。结果表明，Ir1lipo显示对 SGC-7901 的高细胞毒性，IC ₅₀值为 5.8 ± 0.2 μM，而复合物对 A549、HepG2、Bel-7402 和 HeLa 细胞没有细胞毒性。细胞集落表明，负载铱（III）复合物的脂质体可以抑制细胞增殖，诱导细胞周期停滞在G0/G1期。此外，它们还引起自噬，诱导线粒体膜电位降低并增加细胞内活性氧 (ROS) 含量。这些结果表明，包裹脂质体Ir1lipo和Ir2lipo的复合物通过 ROS 介导的线粒体功能障碍和激活 PI3K（磷酸肌醇 3 激酶）/AKT（蛋白激酶 B）信号通路来抑制 SGC-7901 细胞的生长。