MoS₂ nanosheets (MoS₂ NSs), which exhibited peroxidase-like activity, were prepared using the hydrothermal method. The microstructure and element speciation of the MoS₂ NSs were characterized using transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS), respectively. The obtained MoS₂ NSs could catalyze the H₂O₂-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form blue oxidized TMB (ox TMB) with an absorption peak at 652 nm. The effects of MoS₂ NS concentration, incubation time, temperature, and acidity on the color development of TMB were investigated. Under the optimal conditions (1.0 μg mL⁻¹, 40 min, 40 °C, pH 4.0), the increase in absorbance, which was induced by MoS₂ NS-catalyzed TMB oxidation, enabled the detection of H₂O₂ ranging from 2.5 μmol L⁻¹ to 300 μmol L⁻¹. As H₂O₂ is one of the products of glucose oxidase (Gox)-catalyzed glucose oxidation, a colorimetric method was further established for measuring the concentration of glucose. The absorbance response was linearly dependent on glucose concentration of 2.5–200 μmol L⁻¹, with a detection limit of 2.1 μmol L⁻¹. The proposed method was applied to the assay of glucose in urine samples with satisfactory results.

中文翻译：

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使用二硫化钼纳米片作为过氧化物酶模拟酶测定葡萄糖

使用水热法制备了具有过氧化物酶样活性的MoS ₂纳米片 (MoS ₂ NSs)。MoS ₂ NSs的微观结构和元素形态分别使用透射电子显微镜 (TEM) 和 X 射线光电子能谱 (XPS) 进行表征。获得的MoS ₂ NSs可以催化H ₂ O ₂介导的3,3',5,5'-四甲基联苯胺（TMB）氧化形成蓝色氧化的TMB（ox TMB），在652 nm处有吸收峰。研究了MoS ₂ NS浓度、孵育时间、温度和酸度对TMB显色的影响。在最佳条件下（1.0 μg mL ^-1, 40 min, 40 °C, pH 4.0)，由 MoS ₂ NS 催化的 TMB 氧化诱导的吸光度增加，能够检测到 H ₂ O ₂范围从 2.5 μmol L ^-1到 300 μmol L ^-1 . 由于H ₂ O ₂是葡萄糖氧化酶（Gox）催化葡萄糖氧化的产物之一，因此进一步建立了测定葡萄糖浓度的比色法。吸光度响应线性依赖于 2.5–200 μmol L ^{-1 的}葡萄糖浓度，检测限为 2.1 μmol L ^-1。将所提出的方法应用于尿样中葡萄糖的测定，结果令人满意。