We report a probe, LD-FG, for imaging lipid droplet (LD) dynamics by using structure illumination microscopy. LD-FG allows wash-free imaging of LDs, owing to a hydrogen-bond sensitive fluorogenic response. The replacement of photobleached LD-FG with fresh probe molecules from outside the LDs ensures the long-term stability of the imaging. With this buffering fluorogenic probe, fast and unpredictable dynamic processes of LDs can be visualized.

中文翻译：

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通过氢键敏感荧光探针的缓冲策略对脂滴动力学进行稳定的超分辨率成像

我们报告了一种探针LD-FG，用于通过使用结构照明显微镜对脂滴 (LD) 动力学进行成像。由于氢键敏感的荧光反应，LD-FG允许对 LD 进行免洗成像。用来自 LD 外部的新鲜探针分子替换光漂白的LD-FG，确保了成像的长期稳定性。使用这种缓冲荧光探针，可以可视化快速且不可预测的 LD 动态过程。