From the biotechnological viewpoint, the enzymatic disintegration of plant lignocellulosic biomass is a promising goal since it would deliver fermentable sugars for the chemical sector. Cellobiose dehydrogenase (CDH) is a vital component of the extracellular lignocellulose-degrading enzyme system of fungi and has a great potential to improve catalyst efficiency for biomass processing. In the present study, a CDH from a newly isolated strain of the agaricomycete Coprinellus aureogranulatus (CauCDH) was successfully purified with a specific activity of 28.9 U mg−1. This pure enzyme (MW = 109 kDa, pI = 5.4) displayed the high oxidative activity towards β-1–4-linked oligosaccharides. Not least, CauCDH was used for the enzymatic degradation of rice straw without chemical pretreatment. As main metabolites, glucose (up to 165.18 ± 3.19 mg g−1), xylose (64.21 ± 1.22 mg g−1), and gluconic acid (5.17 ± 0.13 mg g−1) could be identified during the synergistic conversion of this raw material with the fungal hydrolases (e.g., esterase, cellulase, and xylanase) and further optimization by using an RSM statistical approach.

中文翻译：

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伞形孢菌Coprinellus aureogranulatus纤维二糖脱氢酶及其在稻草协同转化中的应用

从生物技术的角度来看，植物木质纤维素生物质的酶解是一个很有前途的目标，因为它可以为化学部门提供可发酵的糖。纤维二糖脱氢酶（CDH）是真菌细胞外木质纤维素降解酶系统的重要组成部分，具有提高生物质加工催化剂效率的巨大潜力。在本研究中，成功​​纯化了来自新分离的伞形孢子菌 Coprinellus aureogranulatus (CauCDH) 菌株的 CDH，比活性为 28.9 U mg-1。这种纯酶（MW = 109 kDa，pI = 5.4）对 β-1-4 连接的寡糖显示出高氧化活性。尤其重要的是，CauCDH 用于在没有化学预处理的情况下对稻草进行酶促降解。作为主要代谢物，葡萄糖（高达 165.18 ± 3.19 mg g-1），