当前位置: X-MOL 学术J. Biomater. Sci. Polym. Ed. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Zinc pthalocyanine loaded poly (lactic acid) nanoparticles by double emulsion methodology for photodynamic therapy against 9 L/LacZ gliosarcoma cells
Journal of Biomaterials Science, Polymer Edition ( IF 3.6 ) Pub Date : 2021-10-23 , DOI: 10.1080/09205063.2021.1980359
Benedito Marcio de Oliveira 1 , Jéssica Beatriz Miranda Teodoro 1 , Jéssica Aparecida Ribeiro Ambrósio 1 , Erika Peterson Gonçalves 1 , Milton Beltrame 1 , Luciana Maria Cortez Marcolino 2 , Juliana Guerra Pinto 2 , Juliana Ferreira-Strixino 2 , Andreza Ribeiro Simioni 1
Affiliation  

Abstract

Development delivery systems, such as nanoparticles, represent a growing area in biomedical research. Nanoparticles (NP) were prepared using a double-emulsion method to load zinc(II) phthalocyanine (ZnPc). NP were obtained using poly (lactic acid) (PLA). ZnPc is a second generation of photosensitizer used in photodynamic therapy (PDT). ZnPc loaded PLA nanoparticles (NPLA-ZnPc) were prepared by double-emulsion method, characterized and available in cellular culture. The mean nanoparticle size presented particle size was 384.7 ± 84.2 nm with polydispersity index (PDI) of 0.150 ± 0.015, and the encapsulation efficiency was of 83%. The nanoparticle formulations presented negative zeta potential values (−27.5 ± 1.0 mV), explaining their colloidal stability. ZnPc loaded nanoparticles maintain its photophysical behavior after encapsulation. Photosensitizer release from nanoparticles was sustained over 168 h with a biphasic ZnPc release profile. An in vitro phototoxic effect in range of 80% was observed in 9 L/LacZ gliosarcoma cells at laser light doses (10 J cm−2) with 3.0 µg mL−1 of NPLA-ZnPc. All the physical–chemical, photophysical and photobiological measurements performed allow us to conclude that ZnPc loaded PLGA nanoparticles is a promising drug delivery system for PDT.



中文翻译:

双乳液法负载锌酞菁的聚乳酸纳米粒子用于光动力治疗 9 L/LacZ 胶质肉瘤细胞

摘要

开发交付系统,例如纳米粒子,代表了生物医学研究中一个不断增长的领域。使用双乳液法负载锌 (II) 酞菁 (ZnPc) 制备纳米颗粒 (NP)。NP 是使用聚乳酸 (PLA) 获得的。ZnPc 是用于光动力疗法 (PDT) 的第二代光敏剂。采用双乳液法制备载有 ZnPc 的 PLA 纳米颗粒 (NPLA-ZnPc),对其进行表征并在细胞培养中使用。平均纳米粒径呈现粒径为 384.7 ± 84.2 nm,多分散指数 (PDI) 为 0.150 ± 0.015,包封率为 83%。纳米颗粒制剂呈现负 zeta 电位值 (-27.5 ± 1.0 mV),解释了它们的胶体稳定性。载有 ZnPc 的纳米粒子在封装后保持其光物理行为。纳米粒子的光敏剂释放持续超过 168 小时,具有双相 ZnPc 释放曲线。一个在具有 3.0 µg mL -1 NPLA-ZnPc的激光剂量 (10 J cm -2 ) 下,在 9 L/LacZ 胶质肉瘤细胞中观察到 80% 范围内的体外光毒性效应。进行的所有物理化学、光物理和光生物学测量使我们得出结论,载有 ZnPc 的 PLGA 纳米粒子是一种有前途的 PDT 药物递送系统。

更新日期:2021-10-23
down
wechat
bug