A micro-capillary electrophoresis (μCE) system is one of the widely adopted techniques in the molecular diagnostics and DNA sequencing due to the benefits of high resolution, rapid analysis, and low reagent consumption, but due to the requirements of bulky high-power suppliers and an expensive laser-induced fluorescence detector module, the conventional set-up of μCE system is not adequate for point-of-care (POC) molecular diagnostics. In this study, we constructed a miniaturized and integrated μCE system which can be manipulated by a smartphone. The smartphone not only powers two boost converters and an excited laser, but also controls the relay for the power switch. Moreover, the complementary metal-oxide-semiconductor (CMOS) camera of the smartphone was used for detecting the fluorescence signal of amplicons amplified with reverse transcription-polymerase chain reaction (RT-PCR). We also developed a web-based application so that the raw data of the recorded fluorescence intensity versus the running time can display typical capillary electropherograms on the smartphone. The total size of the hand-held μCE system was 9.6 cm [Width] × 22 cm [Length] × 15.5 cm [Height], and the weight was ∼1 kg, which is suitable for POC DNA testing. In the integrated smartphone-associated μCE system, we could accurately analyze two genes of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), namely N gene and S gene along with two bracket ladders in 6 min to identify SARS-CoV-2. Such an advanced μCE platform can be applied for a variety of on-site molecular diagnostics fields with user-friendliness.

微毛细管电泳 (μCE) 系统具有高分辨率、快速分析和低试剂消耗等优点，是分子诊断和 DNA 测序中广泛采用的技术之一，但由于体积庞大的大功率供应商的要求和昂贵的激光诱导荧光检测器模块，μCE 系统的传统设置不足以进行即时 (POC) 分子诊断。在这项研究中，我们构建了一个可以通过智能手机操作的小型化集成 μCE 系统。智能手机不仅为两个升压转换器和一个激发激光器供电，而且还控制电源开关的继电器。而且，智能手机的互补金属氧化物半导体 (CMOS) 摄像头用于检测通过逆转录聚合酶链反应 (RT-PCR) 扩增的扩增子的荧光信号。我们还开发了一个基于网络的应用程序，以便记录的荧光强度与运行时间的原始数据可以在智能手机上显示典型的毛细管电泳图。手持式μCE系统的总尺寸为9.6 cm [Width] × 22 cm [Length] × 15.5 cm [Height]，重量约为1 kg，适合POC DNA检测。在集成的智能手机相关 μCE 系统中，我们可以准确分析严重急性呼吸系统综合症冠状病毒 2 的两个基因（我们还开发了一个基于网络的应用程序，以便记录的荧光强度与运行时间的原始数据可以在智能手机上显示典型的毛细管电泳图。手持式μCE系统的总尺寸为9.6 cm [Width] × 22 cm [Length] × 15.5 cm [Height]，重量约为1 kg，适合POC DNA检测。在集成的智能手机相关 μCE 系统中，我们可以准确分析严重急性呼吸系统综合症冠状病毒 2 的两个基因（我们还开发了一个基于网络的应用程序，以便记录的荧光强度与运行时间的原始数据可以在智能手机上显示典型的毛细管电泳图。手持式μCE系统的总尺寸为9.6 cm [Width] × 22 cm [Length] × 15.5 cm [Height]，重量约为1 kg，适合POC DNA检测。在集成的智能手机相关 μCE 系统中，我们可以准确分析严重急性呼吸系统综合症冠状病毒 2 的两个基因（SARS-CoV-2 )，即N基因和S基因以及两个支架阶梯在 6 分钟内识别SARS-CoV-2。这种先进的 μCE 平台可以应用于各种现场分子诊断领域，用户友好。