Purpose: Medical halophytes plants are potent sources of bioactive secondary metabolite components used against different diseases. Avicenniamarina one of the typical halophytes plant species used in folk medicine to treat smallpox, rheumatism, and ulcer. Despite the richness of A.marina with polyphenolic, flavonoids, terpenoid, and terpene, contents remain poorly investigated against cancer types. Consequently, to explore the function-composition relationship of A.marina hexane leaves crude extract, the current study designed to investigate the cytotoxicity, apoptotic and antiproliferative impacts on the colon (HCT-116), liver (HepG2), and breast (MCF-7) cancer cell lines.
Materials and Methods: Therefore, the cytotoxicity impact screening carried out by Sulforhodamine-B assay. While, the initiation of the apoptosis evaluated by chromatin condensing, early apoptosis, late apoptosis and the formation and appearance of apoptotic bodies. On the other hand, the flow cytometry used to identify the phase of inhibition where the determined IC₅₀ value used. While, the chemical composition of the hexane extract was detected using liquid chromatography-mass spectrometry/mass spectrometry.
Results: Revealed that hexane extract showed a weak induction of apoptosis despite the formation of apoptotic bodies and the high cell inhibitory effect on all tested cell lines with IC₅₀ values (23.7 ± 0.7, 44.9 ± 0.93, 79.55 ± 0.57) μg/ml on HCT-116, HepG2, and MCF-7, respectively. Furthermore, it showed the ability to inhibit cell cycle in G0/G1 for HCT-116, S phase for HepG2, and MCF-7.
Conclusion: In the light of these results, the current study suggests that A.marina leaves hexane extract may be considered as a candidate for further anticancer drug development investigations.


中文翻译：

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Avicennia marina 植物提取物的抗癌、抗增殖活性


目的：医用盐生植物是用于对抗不同疾病的生物活性次生代谢物成分的有效来源。Avicennia marina是一种典型的盐生植物，在民间医学中用于治疗天花、风湿病和溃疡。尽管A. marina 富含多酚、类黄酮、萜类化合物和萜烯，但针对癌症类型的含量仍然缺乏研究。因此，为了探索A. marina己烷叶粗提物的功能-组成关系，目前的研究旨在调查对结肠 (HCT-116)、肝脏 (HepG2) 和乳房 (MCF-) 的细胞毒性、凋亡和抗增殖作用。 7) 癌细胞系。
材料和方法：因此，通过 Sulforhodamine-B 法进行细胞毒性影响筛选。同时，通过染色质凝聚、早期细胞凋亡、晚期细胞凋亡以及细胞凋亡小体的形成和出现来评价细胞凋亡的开始。另一方面，流式细胞仪用于识别所使用的IC ₅₀值的抑制阶段。同时，使用液相色谱-质谱/质谱法检测己烷提取物的化学成分。
结果：表明己烷提取物显示出微弱的细胞凋亡诱导，尽管形成了凋亡小体，并且对所有测试的细胞系具有高细胞抑制作用，IC ₅₀HCT-116、HepG2 和 MCF-7 上的值 (23.7 ± 0.7、44.9 ± 0.93、79.55 ± 0.57) μg/ml。此外，它显示出抑制 HCT-116 的 G0/G1 期、HepG2 和 MCF-7 的细胞周期的能力。
结论：鉴于这些结果，目前的研究表明，A. marina叶己烷提取物可被视为进一步抗癌药物开发研究的候选者。