Frontiers in Cellular and Infection Microbiology ( IF 5.7 ) Pub Date : 2021-09-14 , DOI: 10.3389/fcimb.2021.585625 Christian A Lux 1, 2 , James J Johnston 2 , Sharon Waldvogel-Thurlow 2 , Camila Dassi 3 , Richard G Douglas 2 , Do-Yeon Cho 4 , Michael W Taylor 1 , Kristi Biswas 2
Chronic rhinosinusitis (CRS) is a globally prevalent inflammatory condition of the paranasal sinuses which severely impairs patients’ quality of life. An animal model of unilateral sinusitis by transient sinus occlusion has been described previously in rabbits. The aim of this study was to characterise the sinusitis rabbit model by investigating temporal and bilateral changes in the bacterial community and mucosal inflammation.
Development of sinusitis was achieved by endoscopically placing Merocel
Endoscopic evaluation showed unilateral inflammation in all animals examined after the 4-week blocking period and at week 14. Notably, inflammatory changes were also seen in the contralateral sinus of all animals at week 4. MRI images demonstrated unilateral sinus opacification at week 4 in two rabbits, and partial unilateral sinus opacification at week 14 in one rabbit only. Histological analyses revealed substantial spatial heterogeneity of mucosal inflammation with inconsistent findings across all animals. No significant differences in mucosal inflammatory markers (such as goblet cell hyperplasia, epithelial denudation and oedema) could be identified between nostrils at week 14. The bacterial community in the rabbit sinuses was heavily dominated by
This study reaffirmed the ability for development of sinusitis without inoculation of any pathogens in a rabbit model. We were able to demonstrate bilateral sinonasal mucosal inflammation, by inducing unilateral sinus blockage, which resulted in significant changes to the sinonasal bacterial community. These findings may explain some of the clinical observations seen in CRS and warrant further research to reveal potential implications for its therapeutic management.
中文翻译:
单边干预兔鼻窦引起双侧炎症和微生物变化
慢性鼻窦炎 (CRS) 是一种全球流行的鼻窦炎性疾病,严重影响患者的生活质量。先前已经在兔子中描述了由暂时性鼻窦阻塞引起的单侧鼻窦炎的动物模型。本研究的目的是通过调查细菌群落和粘膜炎症的时间和双边变化来表征鼻窦炎兔模型。
鼻窦炎的发展是通过内窥镜放置 Merocel 实现的
内窥镜评估显示在 4 周阻滞期后和第 14 周检查的所有动物均出现单侧炎症。值得注意的是,第 4 周所有动物的对侧鼻窦也出现炎症变化。MRI 图像显示第 4 周有两次单侧鼻窦混浊14 周时仅一只兔子出现部分单侧鼻窦混浊。组织学分析揭示了粘膜炎症的大量空间异质性,所有动物的发现不一致。在第 14 周时,在鼻孔之间没有发现粘膜炎症标志物(如杯状细胞增生、上皮剥脱和水肿)的显着差异。兔鼻窦中的细菌群落主要由
该研究重申了在兔模型中无需接种任何病原体即可发生鼻窦炎的能力。我们能够通过诱导单侧鼻窦阻塞来证明双侧鼻窦粘膜炎症,这导致鼻窦细菌群落发生显着变化。这些发现可能解释了在 CRS 中看到的一些临床观察结果,并需要进一步研究以揭示其治疗管理的潜在影响。