The sentinel lymph node (SLN) is the first lymph node into which lymphatic fluid from tumor tissues flows. The development of a highly sensitive probe for detecting SLNs is desired for the lymph node dissection through intraoperative biopsy. We have previously shown that anionic liposomes tend to accumulate in lymph nodes and that macrophage uptake of liposomes contributes to their accumulation. In the present study, we found that among anionic lipids, phosphatidylserine (PS)-containing liposomes were substantially taken up by macrophages. We identified a new lipid composition to improve the SNL-selectivity of liposome accumulation based on Design-of-Experiment. The optimized PS-containing particles were more selectively accumulate to SLN lymph nodes than existing imaging agents indocyanine green. These results indicate the effectiveness of PS-containing anionic particles in SLN imaging.

中文翻译：

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使用阴离子脂质体和透明质酸酶优化前哨淋巴结成像方法

前哨淋巴结（SLN）是肿瘤组织淋巴液流入的第一个淋巴结。通过术中活检进行淋巴结清扫需要开发一种用于检测 SLN 的高灵敏度探针。我们之前已经表明阴离子脂质体倾向于在淋巴结中积聚，并且脂质体的巨噬细胞摄取有助于它们的积聚。在本研究中，我们发现在阴离子脂质中，含有磷脂酰丝氨酸 (PS) 的脂质体被巨噬细胞大量吸收。我们基于实验设计确定了一种新的脂质成分，以提高脂质体积累的 SNL 选择性。优化后的含 PS 颗粒比现有的显像剂吲哚菁绿更有选择性地积聚到 SLN 淋巴结。