Spirulina is one of the most popular microalgae. Thus, it is essential to improve their biomass and macromolecule production. The adequacy of phytohormone supplementation was shown to be efficient in increasing biomass and lipid concentrations in other microalgae cultivation. Therefore, the present study aimed to investigate the influence of sampling frequency on the production of biomass and biomolecules of Spirulina sp. LEB 18 supplemented with trans-zeatin (tZ) and indole-3-acetic acid (IAA). The microalga was cultivated with tZ and IAA at concentrations 0.01, 0.1, 1, and 10 mg L⁻¹, and the sampling frequency was tested daily and every 10 days. Treatments with a lower sampling frequency (every 10 days) and supplementation of 0.01 mg L⁻¹ IAA enhanced the biomass accumulation by 50% (3.2 g L⁻¹) and the lipid content by 58% (26.8%) than that of the experiment without phytohormone and daily sample removal (2.1 g L⁻¹ and 16.9%, respectively). Therefore, we developed a new strategy to improve microalgal production by supplementing phytohormones. The investigation presents a cultivation system that is efficient and easy to implement in industries because it does not require alterations in the operational aspects of the cultivation.

螺旋藻是最受欢迎的微藻之一。因此，必须提高它们的生物量和大分子产量。在其他微藻培养中，植物激素补充剂的充足性在增加生物量和脂质浓度方面是有效的。因此，本研究旨在研究采样频率对螺旋藻生物量和生物分子产生的影响。LEB 18 补充有反式玉米素 (tZ) 和吲哚-3-乙酸 (IAA)。微藻用浓度为0.01、0.1、1和10mg L ^{-1 的}tZ和IAA培养，并且每天和每10天测试采样频率。采样频率较低（每 10 天）和补充 0.01 mg L 的处理^-1 IAA 与没有植物激素和每日样品去除的实验（分别为 2.1 g L ^-1和 16.9% ）相比，增加了 50% (3.2 g L ^-1 )的生物量积累和 58% (26.8%) 的脂质含量）。因此，我们开发了一种通过补充植物激素来提高微藻产量的新策略。调查提出了一种在工业中高效且易于实施的栽培系统，因为它不需要改变栽培的操作方面。