Lead, a highly toxic pollutant, causes numerous health problems and affects nearly all biological systems thus arousing interest in using antioxidants to reduce its toxic effects. Therefore, the undertaken study estimated the influence of cerium oxide nanoparticles (CeO2-NPs) on the lead acetate-induced genotoxicity and inflammation in the kidney and heart tissues of mice. Twenty male mice were randomly divided into negative control and lead acetate and/or CeO2-NPs administrated groups. Comet and diphenylamine assays were conducted to assess the DNA damage and the expression of apoptosis-related genes and inflammatory cytokines were also measured in addition to the estimation of reactive oxygen species (ROS) level. Co-administration of CeO2-NPs significantly reduced the DNA damage and ROS generation caused by lead acetate in the kidney and heart tissues. The co-administration of CeO2-NPs also ameliorated the lead acetate-induced dysregulation in the expression levels of p53, K-ras, interleukin-6, and cyclooxygenase-2 in the kidney and heart. Conclusion: the co-administration of CeO2-NPs suppresses the genotoxicity, inflammation, and ROS generation resulting from lead acetate administration and restoring the genomic DNA integrity; thus, administration of CeO2-NPs is recommended to minimize the lead acetate-induced hazards.

中文翻译：

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急性口服氧化铈纳米颗粒可抑制小鼠肾和心脏组织中醋酸铅诱导的遗传毒性、炎症和 ROS 生成。

铅是一种剧毒污染物，会引起许多健康问题，并影响几乎所有生物系统，因此引起了人们对使用抗氧化剂来减少其毒性作用的兴趣。因此，所进行的研究评估了氧化铈纳米粒子（CeO2-NPs）对醋酸铅诱导的小鼠肾脏和心脏组织的遗传毒性和炎症的影响。20只雄性小鼠随机分为阴性对照组和醋酸铅和/或CeO2-NPs给药组。进行彗星和二苯胺测定来评估 DNA 损伤，除了估计活性氧 (ROS) 水平之外，还测量凋亡相关基因和炎症细胞因子的表达。CeO2-NPs 的共同给药显着减少了肾脏和心脏组织中醋酸铅引起的 DNA 损伤和 ROS 生成。CeO2-NPs 的共同给药还改善了醋酸铅诱导的肾脏和心脏中 p53、K-ras、白细胞介素 6 和环氧合酶 2 表达水平的失调。结论：CeO2-NPs 的共同给药抑制了醋酸铅给药引起的遗传毒性、炎症和 ROS 的产生，并恢复了基因组 DNA 的完整性；因此，建议使用 CeO2-NPs 以尽量减少醋酸铅引起的危害。