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Diffusion Tensor Imaging in Rat Models of Preclinical Diabetic Nephropathy: A Preliminary Study.
Frontiers in Endocrinology ( IF 5.2 ) Pub Date : 2021-08-27 , DOI: 10.3389/fendo.2021.701116
Xiaoyan Hu 1 , Min Kuang 2 , Bo Peng 3 , Yang Yang 3 , Wei Lin 1 , Wenbo Li 1 , Yinghua Wu 4
Affiliation  

Purpose This study aimed to investigate the value of diffusion tensor imaging to assess renal injury in a rat model of preclinical diabetic nephropathy. Methods Twenty-eight male Sprague Dawley rats were divided into two groups: the normal control (NC) group of 10 rats and the diabetic nephropathy (DN) group of 18 rats. Eight weeks after diabetes induction by streptozotocin, 3.0-T magnetic resonance (MR) imaging (b = 0 and 600 s/mm2, 15 diffusion directions) using a 32-channel knee coil was performed. After MR imaging, we measured serum creatinine, and collected double kidney tissues for pathology. The apparent diffusion coefficients(ADC) and fractional anisotropy(FA) values of the renal cortex and medulla were calculated for all kidneys. Physiological parameters, laboratory parameters, and imaging results were compared between the two groups. Results All DN group animals developed hyperglycemia, polyuria, and emaciation. Serum creatinine was not significantly different between the groups (P > 0.05). Urinary albumin at 2, 4, and 8 weeks was higher in the DN group than in the NC group but <20 µg/min (P < 0.05). Pathologically, renal damage in the DN rats was observed. The ADC value was significantly increased in DN animals in the cortex (1.75×10-3mm2/s),medulla(1.53×10-3mm2/s)compared with NC group(cortex, 1.52×10-3mm2/s; medulla,1.35×10-3mm2/s). The FA value was significantly reduced in DN animals in the cortex (0.21),medulla(0.25)compared with NC group(cortex,0.26;medulla,0.3). Conclusions Increased apparent diffusion coefficients and decreased fractional anisotropy values on diffusion tensor imaging were associated with preclinical DN. Diffusion tensor imaging may be useful in early, non-invasive, quantitative detection, and therapy monitoring of DN.

中文翻译:

临床前糖尿病肾病大鼠模型中的扩散张量成像:初步研究。

目的 本研究旨在探讨弥散张量成像在评估临床前糖尿病肾病大鼠模型中肾损伤的价值。方法28只雄性Sprague Dawley大鼠分为两组:正常对照组(NC)10只,糖尿病肾病(DN)组18只。链脲佐菌素诱发糖尿病 8 周后,使用 32 通道膝关节线圈进行 3.0-T 磁共振 (MR) 成像(b = 0 和 600 s/mm2,15 个扩散方向)。磁共振成像后,我们测量了血清肌酐,并收集了双肾组织进行病理检查。计算所有肾脏的肾皮质和髓质的表观扩散系数(ADC)和分数各向异性(FA)值。比较两组的生理参数、实验室参数和影像学结果。结果所有DN组动物均出现高血糖、多尿和消瘦。血清肌酐组间差异无统计学意义(P > 0.05)。DN 组 2、4 和 8 周的尿白蛋白高于 NC 组,但 ​​<20 µg/min (P < 0.05)。在病理学上,观察到DN大鼠的肾损伤。与NC组(皮质,1.52×10-3mm2/s;髓质,1.35 ×10-3mm2/s)。与NC组(皮质,0.26;髓质,0.3)相比,DN动物皮质(0.21)、髓质(0.25)的FA值显着降低。结论 增加的表观扩散系数和减少的扩散张量成像分数各向异性值与临床前 DN 相关。
更新日期:2021-08-27
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