Ammonium sulfate wastewater can cause eutrophication and black odor of water body. Although ammonia nitrogen can be used as nutrient of microalgae, high ammonia nitrogen levels could inhibit the growth of microalgae. Nitrobacteria can transform ammonia nitrogen into nitrate nitrogen. In this study, mono Chlorella pyrenoidosa culture (mono-C.py), synchronous mixed culture (mixed-a), and asynchronous mixed culture (mixed-b) systems were examined for their ability to treat ammonium sulfate wastewater. Nitrogen removal rate of mixed-b at the end of culture (52.96%) was higher than that of the mono-C.py (46.37%) and the mixed-a (39.11%). Higher total suspended solid concentration (2.40 g/L), crude protein yield (0.76 g/L), and heating value yield (35.73 kJ/L) were obtained in mixed-b, meanwhile with excellent settlement performance (91.43 ± 0.51%). Mechanism analysis of settlement showed that the relative abundance of floc-forming-related bacteria Sphingopyxis and Acidovorax were increased generally, while nitrification/denitrifying members were decreased in mixed-b along with the culture proceeding.

硫酸铵废水可引起水体富营养化和黑臭。虽然氨氮可作为微藻的养分，但高氨氮水平会抑制微藻的生长。硝化细菌可以将氨氮转化为硝态氮。在这项研究中，检查了蛋白核小球藻单培养 ( mono-C.py )、同步混合培养 (mixed-a) 和异步混合培养 (mixed-b) 系统处理硫酸铵废水的能力。培养结束时mix -b的脱氮率（52.96%）高于mono-C.py(46.37%) 和混合-a (39.11%)。混合b获得较高的总悬浮物浓度（2.40 g/L）、粗蛋白产率（0.76 g/L）和热值产率（35.73 kJ/L），同时具有优异的沉降性能（91.43±0.51%） . 沉降机理分析表明，随着培养的进行，成絮相关细菌Sphingopyxis和Acidovorax的相对丰度普遍增加，而混合b中硝化/反硝化成员的相对丰度有所减少。