Climate change is predicted to increase the prevalence of vector borne disease due to expansion of insect populations. Candidatus Liberibacter solanacearum (CLso) is a phloem-limited pathogen associated with multiple economically important diseases in Solanaceous crops. Little is known about the strategies and pathogenicity factors CLso uses to colonize vector and host. We determined the CLso effector repertoire by predicting SEC secreted proteins across four different CLso haplotypes, investigated effector localization in planta, and profiled effector expression in vector and host. The localization of CLso effectors in Nicotiana revealed diverse eukaryotic subcellular targets. The majority of tested effectors were unable to suppress plant immune responses, indicating they possess unique activities. Expression profiling in tomato and the psyllid Bactericera cockerelli indicated CLso differentially interacts with its host and vector and can switch effector expression in response to the environment. This study reveals CLso effectors possess complex expression patterns, target diverse host organelles and the majority are unable to suppress host immune responses. A mechanistic understanding of Lso effector function will reveal novel targets and provide insight into phloem biology.

中文翻译：

---

来自细菌载体传播的病原体的效应器表现出不同的亚细胞定位、表达谱和植物防御的操纵

由于昆虫种群的扩大，预计气候变化会增加媒介传播疾病的流行。Candidatus Liberibacter solanacearum ( C Lso) 是一种韧皮部限制性病原体，与茄科作物中多种经济上重要的病害有关。关于C Lso 用于定殖载体和宿主的策略和致病因子知之甚少。我们通过预测 SEC 分泌的蛋白质跨越四种不同的C Lso 单倍型，研究了植物中的效应器定位，并分析了载体和宿主中的效应器表达，从而确定了C Lso 效应器库。C Lso 效应子在烟草中的定位揭示了多种真核亚细胞靶标。大多数测试的效应器无法抑制植物免疫反应，表明它们具有独特的活性。番茄和木虱Bactericera cockerelli中的表达谱表明C Lso 与其宿主和载体有差异的相互作用，并且可以根据环境转换效应子的表达。该研究表明， CLso效应器具有复杂的表达模式，靶向多种宿主细胞器，并且大多数不能抑制宿主免疫反应。对 Lso 效应器功能的机械理解将揭示新的靶点并提供对韧皮部生物学的深入了解。