Gut microbiota and amino acids that are one of their metabolites play important roles in the mechanism of pathology of Parkinson's disease (PD). It has been reported that the level of amino acids in vivo participate in neurodegeneration by regulating adaptive immune response, while the current researches on alteration of amino acids in gut microbiota are still insufficient. We hypothesized that alterations in gut microbiota might be accompanied by altered concentrations of amino acids, leading to the occurrence of PD. In this study, we collected stool samples from PD and healthy controls to analyse fecal microbiome and targeted metabolome by 16S ribosomal RNA (16S rRNA) gene sequencing and gas chromatography coupled to mass spectrometry (GC-MS). At the genus level, there was a greater abundance of Alistipes, Rikenellaceae_RC9_gut_group, Bifidobacterium, Parabacteroides, while Faecalibacterium was decreased in fecal samples from PD patients. Moreover, fecal branched chain amino acids (BCAAs) and aromatic amino acids concentrations were significantly reduced in PD patients compared to controls. Our study not only finds the abundance of certain gut microbiota in PD,but also reveals that it is related to BCAAs and aromatic amino acids. These findings are beneficial to identifying new therapeutic targets for PD by regulating diet and/or gut microbiota.

作为其代谢物之一的肠道微生物群和氨基酸在帕金森病 (PD) 的病理机制中起着重要作用。据报道，体内氨基酸水平通过调节适应性免疫反应参与神经退行性变，而目前对肠道菌群中氨基酸变化的研究还不够。我们假设肠道微生物群的改变可能伴随着氨基酸浓度的改变，导致 PD 的发生。在这项研究中，我们收集了 PD 和健康对照的粪便样本，通过 16S 核糖体 RNA (16S rRNA) 基因测序和气相色谱与质谱联用 (GC-MS) 分析粪便微生物组和靶向代谢组。在属水平上，Alistipes、Rikenellaceae_RC9_gut_group、双歧杆菌、副杆菌属和粪杆菌在 PD 患者的粪便样本中减少。此外，与对照组相比，PD 患者的粪便支链氨基酸 (BCAA) 和芳香族氨基酸浓度显着降低。我们的研究不仅发现了 PD 中某些肠道微生物群的丰度，而且揭示了它与支链氨基酸和芳香族氨基酸有关。这些发现有利于通过调节饮食和/或肠道微生物群来确定 PD 的新治疗靶点。但也揭示了它与支链氨基酸和芳香族氨基酸有关。这些发现有利于通过调节饮食和/或肠道微生物群来确定 PD 的新治疗靶点。但也揭示了它与支链氨基酸和芳香族氨基酸有关。这些发现有利于通过调节饮食和/或肠道微生物群来确定 PD 的新治疗靶点。