Qualitative and quantitative analyses of quercetin (Qu) and isorhamnetin (Is) in Hippophae rhamnoides L. fruits hydrolysis products from 10 different origins were conducted by high-performance thin-layer chromatography (HPTLC), using 3% sodium acetate silica gel G plate (Anhui Liangchen), dichloromethane–ethyl acetate–formic acid (7:3:1, V/V) as the developing reagent, 1% ethanol solution of aluminum trichloride as the chromogenic agent, scanning at a wavelength of 390 nm. The content of Qu and Is in H. rhamnoides L. fruits hydrolysis products from 10 different producing areas was determined. The results showed that the R_F value of Qu was 0.48 ± 0.05, the linear range was 0.06–0.42 μg/μL, and the correlation coefficient was 0.9990, indicating that the linear correlation of Qu was good. The R_F value of Is was 0.64 ± 0.05, the linear range was 0.04–0.44 μg/μL, and the correlation coefficient was 0.9993, indicating that the linear correlation of Is was good. The precision analyses of Qu and Is showed that the RSD was < 5.0%, indicating the good precision of the instrument. The repeatability analyses of both Qu and Is showed an RSD < 3.0%, indicating that the method is reproducible. The limit of detection (LOD) of Qu was 50 ng, and the limit of quantification (LOQ) was 165 ng. The LOD of Is was 50 ng, and the LOQ was 167 ng. The stability study showed that the sample was stable within 90 min at room temperature, with an RSD < 2.0%. The average recovery of Qu was 99.99%; the average recovery of Is was 100.79%. The results showed that the Is content was higher than the Qu content in the Inner Mongolia and Shanxi origins, and the Qu content was higher than the Is content in the eight other origins.

使用 3% 醋酸钠硅胶 G 板，通过高效薄层色谱法 (HPTLC) 对 10 个不同产地的沙棘果实水解产物中槲皮素 (Qu) 和异鼠李素 (Is) 进行定性和定量分析。安徽良辰），二氯甲烷-乙酸乙酯-甲酸（7：3：1，V/V）为展开剂，1%三氯化铝乙醇溶液为显色剂，扫描波长390nm。测定了来自10个不同产区的H. rhamnoides L.水果水解产物中Qu和Is的含量。该结果表明，[R _˚FQu 值为 0.48±0.05，线性范围为 0.06～0.42 μg/μL，相关系数为 0.9990，说明 Qu 的线性相关性良好。在[R _˚FIs值为0.64±0.05，线性范围为0.04～0.44 μg/μL，相关系数为0.9993，表明Is线性相关性良好。Qu 和 Is 的精密度分析表明 RSD < 5.0%，表明仪器精密度良好。Qu 和 Is 的重复性分析显示 RSD < 3.0%，表明该方法具有重现性。Qu 的检测限 (LOD) 为 50 ng，定量限 (LOQ) 为 165 ng。Is 的 LOD 为 50 ng，LOQ 为 167 ng。稳定性研究表明，样品在室温下 90 分钟内稳定，RSD < 2.0%。曲的平均回收率为99.99%；Is的平均回收率为100.79%。结果表明，内蒙古和山西地区的Is含量高于Qu含量，