Mimicking the natural bone extracellular matrix containing intrinsic topography and electrical signals is an effective way to modulate bone regeneration. However, simultaneously coupling of the intrinsic mechanobiology and electrical cues of implant to modulate bone regeneration remains ignored. Here, the authors report in situ designation of titanium dioxide (TiO₂) nanocone/bismuth oxide (Bi₂O₃) nanodot heterojunctions on bone implant surface to electro-biomechanically trigger osseointegration at bone/implant interface. TiO₂ nanocone/Bi₂O₃ nanodot heterojunctions exhibit built-in electric field at the nanoscale interface and elastic modulus equivalent to that of bone tissue. The nano-heterojunctions significantly promoted the attachment, spreading, and osteogenic differentiation of bone marrow mesenchymal stem cells in vitro, and the osteogenesis in vivo. The authors also show that the effects of nano-heterojunctions on osteogenesis are mediated by yes-associated protein biomechanical signal pathway and intracellular enrichment induced Phosphatidylinositol 3-kinase signal pathway. Their findings highlight the coupling of topographical and electric parameters of biomaterials for modulating cell behaviors.

中文翻译：

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具有机电生物耦合线索的 0D/1D 异质结植入物促进成骨

模仿包含内在地形和电信号的天然骨细胞外基质是调节骨再生的有效方法。然而，同时耦合植入物的内在机械生物学和电线索以调节骨再生仍然被忽略。在这里，作者报告了在骨植入物表面原位指定二氧化钛 (TiO ₂ )纳米锥/氧化铋 (Bi ₂ O ₃ ) 纳米点异质结，以电生物力学触发骨/植入物界面的骨整合。TiO ₂纳米锥/Bi ₂ O ₃纳米点异质结在纳米级界面处表现出内置电场，弹性模量与骨组织相当。纳米异质结显着促进了体外骨髓间充质干细胞的附着、扩散和成骨分化，以及体内的成骨。作者还表明，纳米异质结对成骨的影响是由 yes 相关蛋白生物力学信号通路和细胞内富集诱导的磷脂酰肌醇 3-激酶信号通路介导的。他们的发现强调了生物材料的拓扑参数和电参数的耦合以调节细胞行为。