Quartz crystal microbalance (QCM) sensor and bioactive-modified thin films of polystyrene (PS) have been successfully and respectively fabricated using resistance evaporation technique. The films’ quality, bovine serum albumin (BSA) adsorption characteristic, and sensor performance were investigated. Firstly, the aluminum and silver layers were, respectively, sputtered on the At-cut quartz to form QCM sensors and characterized by atomic force microscopy. The aluminum layer exhibits much greater roughness than silver layer. The QCM sensor coated with silver electrode layer of 300 nm exhibits the resonance frequency around 10 MHz and more superior Q-factor around 8000. Then, the morphology of the deposited PS membranes were analyzed by positive fluorescence microscope, attenuated total reflectance Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and water contact angle methods. The island-like PS membranes were achieved and used as sensitive layer for BSA adsorption. A ~ 186 Hz drop in the PS-based QCM sensor resonance frequency was observed when the sensor exposed to the BSA solution that dissolved in pure phosphate buffer saline. Finally, the QCM sensor has good linear sensitivity of ~ 87 Hz/(100 μg/mL) to different BSA concentrations from 50 μg/mL to 500 μg/mL.

石英晶体微量天平 (QCM) 传感器和聚苯乙烯 (PS) 生物活性改性薄膜已分别使用电阻蒸发技术成功制造。研究了薄膜的质量、牛血清白蛋白 (BSA) 吸附特性和传感器性能。首先，将铝层和银层分别溅射在切割石英上以形成 QCM 传感器，并通过原子力显微镜进行表征。铝层表现出比银层大得多的粗糙度。涂有 300 nm 银电极层的 QCM 传感器表现出大约 10 MHz 的共振频率和更高的Q-因子约为8000。然后，通过正荧光显微镜、衰减全反射傅里叶变换红外光谱、X射线光电子能谱和水接触角方法分析沉积的PS膜的形貌。获得岛状 PS 膜并将其用作 BSA 吸附的敏感层。当传感器暴露于溶解在纯磷酸盐缓冲盐水中的 BSA 溶液时，观察到基于 PS 的 QCM 传感器共振频率下降约 186 Hz。最后，QCM 传感器对从 50 μg/mL 到 500 μg/mL 的不同 BSA 浓度具有 ~ 87 Hz/(100 μg/mL) 的良好线性灵敏度。