Brucellosis is a zoonotic disease with a global impact. Brucella suis is one of the most pathogenic species to humans, requiring different measures for the control and/or eradication of the disease. The serological investigation for brucellosis was performed in pigs, horses, dogs, and cattle on a farm with a history of abortion in sows and necropsy of a boar with severe necrosuppurative orchitis. One sow, two cows, and two dogs reveled positive to Rose Bengal Test (RBT), although only the sow had a confirmatory outcome in 2-mercaptoethanol (2-ME). The 2-ME-positive sow was euthanized and microbiological culture of lymph nodes and liver followed by biochemical characterization allowed phenotypic characterization of Brucella suis biotype 1. PCR multiplex Bruce-ladder and Suis-ladder enabled molecular confirmation, respectively, of Brucella suis and biotype 1. The transmission aspects of B. suis to pigs and other domestic species, the combination of diagnostic procedures to diagnosis, as well as human health concerns of brucellosis are discussed.

布鲁氏菌病是一种具有全球影响的人畜共患病。猪布鲁氏菌 是对人类最具致病性的物种之一，需要采取不同的措施来控制和/或根除这种疾病。布鲁氏菌病的血清学调查是在一个有母猪流产史的农场的猪、马、狗和牛身上进行的，并对一头患有严重坏死性化脓性睾丸炎的公猪进行尸检。一只母猪、两只母牛和两只狗对玫瑰红试验 (RBT) 呈阳性反应，尽管只有母猪在 2-巯基乙醇 (2-ME) 中得到了确认结果。对 2-ME 阳性母猪实施安乐死，对淋巴结和肝脏进行微生物培养，然后进行生化鉴定，从而对猪布鲁氏菌进行表型鉴定 生物型 1. PCR 多重 Bruce-ladder 和Suis -ladder 分别实现了猪布鲁氏菌 和生物型 1 的分子确认。讨论了布鲁氏菌病的人类健康问题。