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The influence of pre-treatment methods and matrix effect on sesame (Sesamum indicum) sandwich ELISA detection
Food and Agricultural Immunology ( IF 3 ) Pub Date : 2021-09-13 , DOI: 10.1080/09540105.2021.1973967
Junyu Wang 1 , Wenjie Li 2 , Yaqing Wang 3 , Tushar Pavase 1 , Jiukai Zhang 4 , Zhenxing Li 1 , Hong Lin 1
Affiliation  

ABSTRACT

Food allergy is a serious health risk and severely affects the quality of life in individuals all over the globe. In recent years, there has been an evident increase in the number of food allergy cases due to the consumption of sesame. Hence, to develop a robust and reliable detection method for sesame allergen has become a high priority. In this research, a sandwich ELISA method was established to detect sesame allergens using rat and rabbit antiserums. The limit of detection for sandwich ELISA was estimated at 1 ng/mL, revealing high sensitivity. At the same time, it was found that the most suitable pre-treatment extract for this method was 0.1 M, pH 9.6 Na2CO3-NaHCO3 containing 0.5% SDS. This sandwich ELISA method can sensitively and efficiently detect foods containing sesame ingredients, which can protect the sensitive individuals from the exposure to sesame allergenic foods.



中文翻译:

前处理方法和基质效应对芝麻(Sesamum indicum)夹心ELISA检测的影响

摘要

食物过敏是一种严重的健康风险,严重影响全球个人的生活质量。近年来,因食用芝麻而引起的食物过敏病例明显增多。因此,开发一种稳健可靠的芝麻过敏原检测方法已成为当务之急。本研究建立了夹心ELISA方法,利用大鼠和兔抗血清检测芝麻过敏原。夹心 ELISA 的检测限估计为 1 ng/mL,显示出高灵敏度。同时,发现最适合该方法的预处理提取物为 0.1 M,pH 9.6 Na 2 CO 3 -NaHCO 3含有 0.5% SDS。这种夹心ELISA方法可以灵敏、高效地检测出含有芝麻成分的食品,可以保护敏感人群免受芝麻致敏食品的影响。

更新日期:2021-09-13
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