Rhizoctonia root rot is an important bean disease that causes significant yield losses. Increasing the levels of plant resistance using biological and chemical inducers is a sustainable strategy for plant disease management. In this study, the efficiency of thiamine, riboflavin, pyridoxine, folic acid, menadione, quercetin, naringenin, humic acid, fulvic acid, boric acid, zinc sulfate, manganese sulfate, Piriformospora indica and binucleate Rhizoctonia were evaluated to improve bean resistance to root rot pathogen Rhizoctonia solani AG-4 HGII. Under laboratory conditions, effect of various biological agents and chemical compounds on mycelial growth of the pathogen was evaluated. Results showed that applying boric acid, zinc sulfate and manganese sulfate at 1000 and 2000 mg L⁻¹ concentrations and menadione at 2000, 5000, 10000 and 20000 μM completely inhibited the pathogen growth and 10000 and 20000 μM concentrations of thiamine reduced linear growth of R. solani. Under greenhouse conditions, application of the inducers significantly reduced the disease progress on treated plants compared to the non-treated plants. The highest level of disease reduction was observed using zinc sulfate and thiamine applied at 3 days before the pathogen inoculation, and also by using P. indica 14 days before inoculation with the pathogen. Thiamine, zinc sulfate and P. indica significantly reduced the disease index and improved plant growth parameters compared to the controls. Induction of resistance against R. solani by individual and combined treatments using thiamine, zinc sulfate and P. indica was associated with increased activity of various antioxidants and increased levels of membrane stability index (MSI), relative water content (RWC), lignin accumulation and total soluble phenolics in bean seedlings. Our findings revealed a practical possibility of inducing resistance and protection of bean plants against Rhizoctonia root rot with the help of biological and chemical resistance inducers.

丝核菌根腐病是一种重要的豆类病害，会导致显着的产量损失。使用生物和化学诱导剂提高植物抗性水平是植物病害管理的可持续策略。在这项研究中，硫胺素的EF法fi ciency，核黄素，吡哆醇，叶酸，甲萘醌，槲皮素，柚皮素，腐殖酸，灰黄霉酸，硼酸，硫酸锌，硫酸锰，Piriformospora籼稻和双核丝核菌进行了评价，以改善到根bean电阻腐烂病原丝核菌AG-4 HGII。在实验室条件下，评估了各种生物制剂和化合物对病原体菌丝体生长的影响。结果表明，施用硼酸，硫酸锌和硫酸锰在1000和2000毫克的L ^-1的浓度和甲萘醌在2000，5000，10000和20000μM完全抑制病原体生长和硫胺素10000个20000μM浓度降低的线性增长ř 。菌。在温室条件下，与未经处理的植物相比，诱导剂的应用显着降低了经处理植物的疾病进展。在病原体接种前 3 天使用硫酸锌和硫胺素以及使用P. indica观察到的疾病减少水平最高接种病原体前14天。与对照相比，硫胺素、硫酸锌和P. indica显着降低了疾病指数并改善了植物生长参数。通过使用硫胺素、硫酸锌和P. indica的单独和组合处理诱导对R. solani的抗性与各种抗氧化剂的活性增加和膜稳定性指数 (MSI)、相对含水量 (RWC)、木质素积累和豆苗中的总可溶性酚类物质。我们的研究结果揭示了在生物和化学抗性诱导剂的帮助下诱导抗性和保护豆类植物免受丝核菌根腐病的实际可能性。