Long non-coding RNA DLX6 antisense RNA 1 (DLX6-AS1) lists a critical position in thyroid carcinoma (TC) development. However, the overall comprehension about DLX6-AS1, microRNA (miR)-193b-3p and homeobox A1 (HOXA1) in TC is not thoroughly enough. Concerning to this, this work is pivoted on DLX6-AS1/miR-193b-3p/HOXA1 axis in TC cell growth and autophagy. TC tissues and adjacent normal thyroid tissues were collected, in which expression of DLX6-AS1, miR-193b-3p and HOXA1 was tested, together with their interactions. TC cells were transfected with DLX6-AS1/miR-193b-3p-related oligonucleotides or plasmids to test cell growth and autophagy. Tumorigenesis in nude mice was observed. DLX6-AS1 and HOXA1 were up-regulated, and miR-193b-3p was down-regulated in TC. Depleted DLX6-AS1 or restored miR-193b-3p disturbed cell growth and promoted autophagy. DLX6-AS1 targeted miR-193b-3p and positively regulated HOXA1. miR-193b-3p inhibition mitigated the impaired tumorigenesis induced by down-regulated DLX6-AS1. Tumorigenesis in nude mice was consistent with that in cells. It is clear that DLX6-AS1 depletion hinders TC cell growth and promotes autophagy via up-regulating miR-193b-3p and down-regulating HOXA1.

中文翻译：

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沉默长链非编码 RNA DLX6-AS1 或恢复 microRNA-193b-3p 通过抑制 HOXA1 增强甲状腺癌细胞自噬和凋亡

长链非编码 RNA DLX6 反义 RNA 1 (DLX6-AS1) 列出了甲状腺癌 (TC) 发展中的关键位置。然而，对TC中的DLX6-AS1、microRNA（miR）-193b-3p和同源框A1（HOXA1）的整体理解还不够透彻。关于这一点，这项工作以 TC 细胞生长和自噬中的 DLX6-AS1/miR-193b-3p/HOXA1 轴为中心。收集TC组织和邻近的正常甲状腺组织，检测DLX6-AS1、miR-193b-3p和HOXA1的表达及其相互作用。用 DLX6-AS1/miR-193b-3p 相关寡核苷酸或质粒转染 TC 细胞以测试细胞生长和自噬。观察到裸鼠的肿瘤发生。DLX6-AS1 和 HOXA1 在 TC 中上调，miR-193b-3p 下调。耗尽 DLX6-AS1 或恢复 miR-193b-3p 会干扰细胞生长并促进自噬。DLX6-AS1 靶向 miR-193b-3p 并正向调节 HOXA1。miR-193b-3p 抑制减轻了由下调的 DLX6-AS1 诱导的受损肿瘤发生。裸鼠的肿瘤发生与细胞一致。很明显，DLX6-AS1 耗竭会阻碍 TC 细胞生长并通过上调 miR-193b-3p 和下调 HOXA1 促进自噬。