Background

Mesenchymal stem cells-derived adipose tissue (AT-MSCs) are recognized for the treatment of inflammatory diseases including multiple sclerosis (MS). Hypericum perforatum (HP) is an anti-inflammatory pharmaceutical plant with bioactive compounds. Plant tissue culture is a technique to improve desired pharmacological potential. The aim of this study was to compare the anti-inflammatory and proliferative effects of callus with field-growing plant extracts of HP on AT-MSCs derived from MS patients.

Materials and methods

AT-MSCs were isolated and characterized. HP callus was prepared and exposure to light spectrum (blue, red, blue-red, and control). Total phenols, flavonoids, and hypericin of HP callus and plant extracts were measured. The effects of HP extracts concentrations on proliferation were evaluated by MTT assay. Co-culture of AT-MSCs: PBMCs were challenged by HP plant and callus extracts, and Tregs percentage was assessed by flow cytometry.

Results

Identification of MSCs was performed. Data showed that blue light could stimulate total phenols, flavonoids, and hypericin. MTT test demonstrated that plant extract in concentrations (0.03, 1.2, 2.5 and 10 μg/ml) and HP callus extract in 10 μg/ml significantly increased. Both HP extracts lead to an increase in Tregs percentage in all concentrations. In particular, a comparison between HP plant and callus extracts revealed that Tregs enhanced 3-fold more than control groups in the concentration of 10 μg/ml callus.

Conclusions

High concentrations of HP extracts showed effectiveness on AT-MSCs proliferation and immunomodulatory properties with a certain consequence in callus extract. HP extracts may be considered as supplementary treatments for the patients who receiving MSCs transplantation.

中文翻译：

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贯叶连翘植物和愈伤组织提取物对多发性硬化症患者间充质干细胞来源脂肪组织增殖和免疫调节潜力的比较评估

背景

间充质干细胞来源的脂肪组织 (AT-MSCs) 被公认为可用于治疗包括多发性硬化症 (MS) 在内的炎症性疾病。Hypericum perforatum (HP) 是一种具有生物活性化合物的抗炎药用植物。植物组织培养是一种提高所需药理潜力的技术。本研究的目的是比较愈伤组织与大田生长的 HP 植物提取物对 MS 患者来源的 AT-MSCs 的抗炎和增殖作用。

材料和方法

分离和表征 AT-MSC。制备 HP 愈伤组织并暴露于光谱（蓝色、红色、蓝红色和对照）。测量了 HP 愈伤组织和植物提取物的总酚、类黄酮和金丝桃素。通过MTT法评估HP提取物浓度对增殖的影响。AT-MSCs 的共培养：PBMCs 受到 HP 植物和愈伤组织提取物的攻击，并通过流式细胞术评估 Tregs 百分比。

结果

进行了MSC的鉴定。数据显示，蓝光可以刺激总酚、类黄酮和金丝桃素。MTT 测试表明浓度（0.03、1.2、2.5 和 10 μg/ml）的植物提取物和 10 μg/ml 的 HP 愈伤组织提取物显着增加。两种 HP 提取物均导致所有浓度的 Tregs 百分比增加。特别是，HP 植物和愈伤组织提取物之间的比较显示，在 10 μg/ml 愈伤组织的浓度下，Tregs 的增强比对照组高 3 倍。

结论

高浓度的 HP 提取物对 AT-MSCs 增殖和免疫调节特性显示出有效性，并对愈伤组织提取物产生一定的影响。HP 提取物可作为接受 MSCs 移植患者的补充治疗。