The objective of this study was to observe the effects of anthocyanin from purple corn on blood biochemical indexes, ruminal fluid fermentation parameters, and the microbial population in goats. A total of 18 Qianbei Ma wether kids (body weight, 21.38 ± 1.61 kg; mean ± standard deviation) were randomly assigned to three groups using a completely randomized design. The group diets were: (1) control, basal diet, (2) treatment 1 (LA), basal diet with 0.5-g/d purple corn pigment (PCP), and (3) treatment 2 (HA), basal diet with 1-g/d PCP. The results showed that supplementation of PCP anthocyanin increased (P < 0.05) crude protein and gross energy digestibilities compared to the control. Compared to the control group, the inclusion of anthocyanin-rich PCP led to significantly increased (P < 0.05) plasma reduced glutathione and peroxidase concentrations. Goats receiving PCP had increased (P < 0.05) ruminal fluid acetic acid and a higher ratio of acetate to propionate, while the propionic acid, butyric acid, valeric acid, isobutyric acid, and isovaleric acid levels had decreased (P < 0.05). There was no significant difference (P > 0.05) in ruminal fluid alpha bacterial diversity among the three groups. At the phylum level, the feeding of PCP had significant effect (P < 0.05) on the abundances of Actinobacteriota, Proteobacteria, Elusimicrobiota, WPS-2, and Cyanobacteria. At the genus level, HA group had lower (P < 0.05) Prevotellaceae_NK3B31_group abundance compared to the other groups. In addition, significant differences (P < 0.05) were also observed for the ruminal fluid Eubacterium_nodatum_group, Amnipila, Ruminiclostridium, U29-B03, unclassified_c_Clostridia, Pyramidobacter, Anaeroplasma, UCG-004, Atopobium, norank_f_norank_o_Bradymonadales, Elusimicrobium, norank_f_norank_o_norank_c_norank_p_WPS-2, norank_f_Bacteroidales_UCG-001, and norank_f_norank_o_Gastranaerophilales among all groups. Taken together, the inclusion of anthocyanin-rich PCP increased the antioxidant potential, improved rumen volatile fatty acids, and induced a shift in the structure and relative abundance of ruminal microbiota in growing goats.

本研究的目的是观察紫玉米花青素对山羊血液生化指标、瘤胃液发酵参数和微生物种群的影响。使用完全随机化设计将总共 18 名千北马天气儿童（体重，21.38 ± 1.61 kg；平均值 ± 标准差）随机分配到三组。组日粮为：(1) 对照，基础日粮，(2) 处理 1 (LA)，含有 0.5-g/d 紫玉米色素 (PCP) 的基础日粮，和 (3) 处理 2 (HA)，含有1 克/天 PCP。结果表明，添加五氯苯酚花青素增加了（磷< 0.05) 与对照相比，粗蛋白和总能量消化率。与对照组相比，包含富含花青素的五氯苯酚导致显着增加（磷< 0.05) 血浆还原型谷胱甘肽和过氧化物酶浓度。接受五氯苯酚的山羊增加了（磷 < 0.05) 瘤胃液乙酸和较高的乙酸与丙酸比，而丙酸、丁酸、戊酸、异丁酸和异戊酸水平下降（磷< 0.05)。没有显着差异（磷> 0.05) 三组间瘤胃液中α细菌的多样性。在门水平上，饲喂五氯苯酚有显着影响（磷 < 0.05) 的丰度 放线菌群、变形菌群、Elusimicrobiota、WPS-2 和 蓝藻. 在属水平上，HA组具有较低的（磷 < 0.05) 普氏菌科_NK3B31_group 丰度与其他组相比。此外，显着差异（磷 < 0.05) 也观察到瘤胃液 真细菌_nodatum_group， Amnipila, 瘤胃梭菌, U29-B03, unclassified_c_梭状芽孢杆菌、锥状杆菌属、无形体, UCG-004, 奇异果, norank_f_norank_o_Bradymonadales, Elusimicrobium, norank_f_norank_o_norank_c_norank_p_WPS-2, norank_f_拟杆菌目_UCG-001 和 norank_f_norank_o_嗜胃菌在所有群体中。总之，添加富含花青素的五氯苯酚提高了抗氧化能力，改善了瘤胃挥发性脂肪酸，并诱导了生长山羊瘤胃微生物群结构和相对丰度的变化。