The present study deals with the evaluation of the age-defying potential of topical cream formulations bearing Geranium essential oil/Calendula essential oil-entrapped ethanolic lipid vesicles (ELVs). Two types of cream formulations were prepared, viz., conventional and ELVs spiked o/w creams. Essential oil- (EO-) loaded ELVs were characterized by vesicle size, polydispersity index, encapsulation efficiency, and scanning electron microscopy. The cream formulations were evaluated for homogeneity, spreadability, viscosity, pH, in vitro antioxidant capacity, sun protection factor, and in vitro collagenase and elastase inhibition capacity. Confocal laser scanning microscopy (CLSM) was performed to ascertain skin permeation of conventional and vesicular cream. The results of in vitro antioxidant studies showed that GEO-/CEO-loaded vesicular creams have notable antioxidant capacity when compared to nonvesicular creams. GEO- or CEO-loaded vesicular creams exhibited the highest SPF value 10.26 and 18.54, respectively. Both the EO-based vesicular creams showed in vitro collagenase and elastase enzyme inhibition capacity. CLSM images clearly depicted that vesicular cream deep into the skin layers. From the research findings, the age-defying potential and photoprotective effects of GEO and CEO were confirmed. It can be concluded that ELVs are able to preserve the efficiency of EOs and have the potential to combat skin aging.

中文翻译：

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外用天竺葵/金盏花精油包埋乙醇脂质囊泡霜对抗皮肤老化

本研究涉及评估含有天竺葵精油/金盏花精油包埋的乙醇脂质囊泡 (ELV) 的外用乳膏配方的抗衰老潜力。制备了两种类型的乳膏配方，即常规乳膏和添加 ELV 的 o/w 乳膏。精油 (EO-) 负载 ELV 的特点是囊泡大小、多分散指数、封装效率和扫描电子显微镜。评估了乳膏配方的均匀性、涂抹性、粘度、pH、体外抗氧化能力、防晒系数以及体外胶原酶和弹性蛋白酶抑制能力。进行共聚焦激光扫描显微镜 (CLSM) 以确定常规和水泡霜的皮肤渗透性。结果体外抗氧化研究表明，与非囊泡霜相比，含有 GEO/CEO 的囊泡霜具有显着的抗氧化能力。载有 GEO 或 CEO 的水泡霜的 SPF 值最高，分别为 10.26 和 18.54。两种基于 EO 的囊泡霜均显示出体外胶原酶和弹性蛋白酶抑制能力。CLSM 图像清楚地描绘了深入皮肤层的水泡霜。研究结果证实了 GEO 和 CEO 的抗衰老潜力和光保护作用。可以得出结论，ELVs 能够保持 EOs 的效率，并具有对抗皮肤老化的潜力。