Alkaline sulfite pretreated sugarcane bagasse was enzymatically hydrolyzed in a packed-bed column reactor and a bubble column reactor was evaluated to produce ethanol from the hydrolysate. Initial solid loadings of 9–16% were used in column reactor in the hydrolysis step, and the use of lower value (9%) resulted in 41 g L⁻¹ of glucose in the hydrolysate, corresponding to 87% of cellulose hydrolysis yield. This yield was reduced to 65% for a solid loading of 16%, corresponding to a glucose concentration of 54 g L⁻¹. Subsequently, Saccharomyces cerevisiae and Scheffersomyces stipitis were used for ethanol production in medium based on hydrolysate previously obtained, using different aeration flowrates (0.3, 0.5 and 0.7 vvm). In simple batch fermentation using S. cerevisiae, higher ethanol yield (0.40 g.g⁻¹) and productivity (1.58 g.L⁻¹.h⁻¹) were achieved using 0.5 vvm. When S. stipitis was used in simple batch co-fermentations, the maximum ethanol productivities were obtained using 0.5 and 0.7 vvm (0.64 and 0.63 g.L⁻¹.h⁻¹, respectively). Successive repeated batches resulted in average ethanol concentration of 38 g.L⁻¹ and fermentation efficiency of 82%, when using S. cerevisiae. For S. stipitis, those values were, respectively, 36 g.L⁻¹ and 50%, with volumetric productivity increased along the cycles. Thus, the potential of the bioreactors as simple systems for use in the biological steps of biorefineries was demonstrated.

碱性亚硫酸盐预处理的甘蔗渣在填充床反应器中进行酶水解，并评估气泡塔反应器从水解产物中生产乙醇。在水解步骤中，柱反应器中使用的初始固含量为 9-16%，使用较低的值 (9%) 导致水解产物中的葡萄糖为 41 g L ^-1，相当于纤维素水解产率的 87%。对于 16% 的固体负载，该产率降低至 65%，对应于 54 g L ^-1的葡萄糖浓度。随后，Saccharomyces cerevisiae和Scheffersomyces stipitis用于在基于先前获得的水解产物的培养基中使用不同的曝气流速（0.3、0.5 和 0.7 vvm）生产乙醇。在使用酿酒酵母的简单分批发酵中，使用 0.5 vvm 可获得更高的乙醇产量（0.40 gg ^-1）和生产力（1.58 gL ^-1 .h ^{-1 ）。}当树干链球菌用于简单的批量共发酵时，使用 0.5 和 0.7 vvm（分别为 0.64 和 0.63 gL ^-1 .h ^-1）获得了最大的乙醇产率。当使用酿酒酵母时，连续重复批次导致平均乙醇浓度为 38 gL ^-1和发酵效率为 82% 。为了S. stipitis，这些值分别为 36 gL ^-1和 50%，随着循环的体积生产力增加。因此，证明了生物反应器作为用于生物精炼厂的生物步骤的简单系统的潜力。