Henipaviruses, Hendra (HeV) and Nipah (NiV), are highly pathogenic zoonotic agents that pose a serious health risk to human life, and as such are restricted to physical containment 4 (PC4) laboratories. For further analysis of virus-infected biological specimens, it is necessary to ensure absolute inactivation of any infectious virus present before removal from the PC4 laboratory. To evaluate the inactivation of HeV and NiV within infected samples, two chemical inactivation methods were assessed. Henipavirus-infected cell monolayers treated with 4 % paraformaldehyde (PFA) showed the complete inactivation of infectious virus, with an inactivation period of 15 min resulting in more than 8-log decrease in infectious titre. NiV-infected tissue samples treated with 10 % neutral-buffered formalin (NBF) showed a complete reduction of infectious virus in 7/8 ferret organs incubated for 24 h, with the remaining tissue demonstrating complete virus inactivation after 48 h. The chemical inactivation methods described herein evaluated two simple methods of henipavirus inactivation, resulting in the complete inactivation of infectious virus – an essential requirement for the safe removal and handling of biological samples from the PC4 laboratory.

Henipaviruses、Hendra (HeV) 和 Nipah (NiV) 是高致病性人畜共患病病原体，对人类生命构成严重的健康风险，因此仅限于物理遏制 4 (PC4) 实验室。为了进一步分析受病毒感染的生物样本，有必要确保在从 PC4 实验室中取出之前存在的任何感染性病毒绝对灭活。为了评估感染样本中 HeV 和 NiV 的灭活，评估了两种化学灭活方法。用 4% 多聚甲醛 (PFA) 处理的亨尼帕病毒感染细胞单层显示感染性病毒完全灭活，灭活期为 15 分钟，导致感染滴度降低 8-log 以上。用 10% 中性缓冲福尔马林 (NBF) 处理的 NiV 感染组织样本显示，在 7/8 雪貂器官中孵育 24 小时后传染性病毒完全减少，剩余组织在 48 小时后显示病毒完全灭活。本文描述的化学灭活方法评估了两种简单的亨尼帕病毒灭活方法，从而导致传染性病毒的完全灭活——这是从 PC4 实验室安全移除和处理生物样品的基本要求。