Nano-zero-valent iron (nZVI), as a typical nano-material, has been recently used in wastewater treatment and combination with bioreactors. Using nZVI coupled denitrification system research the effect and influence of nZVI enhanced denitrification sludge on the degradation of toxic compounds and system performance. The nZVI coupled denitrification system showed better resistance to 2,6-DCP impact, and the concentrations of effluent NO₂⁻ and NO₃⁻ were below 2.0 mg/L. At the same time, the addition of nZVI enabled the denitrification system to quickly adapt to the toxic environment of 2,6-DCP within 15 days, and the degradation efficiency of 2,6-DCP reached 99.9%. The released SMP reduced after nZVI coupled with denitrification sludge in 2,6-DCP environment, which could improve the effluent water quality. Nuclear magnetic resonance spectroscopy showed that the addition of nZVI would change the structure of EPS in denitrification sludge. After 90 days of operation, the dominant bacteria in the denitrifying sludge have undergone great changes. Moreover, Thauera was responsible as the dominant bacteria for degrading 2,6-DCP in the denitrification system. The increased in the proportion of functional bacteria with nitrate_reduction, nitrogen_respiration, nitrate_respiration and nitrite_respiration in the presence of NZVI further reveals the mechanism of enhanced denitrification.

纳米零价铁（nZVI）作为一种典型的纳米材料，最近被用于废水处理和与生物反应器的结合。利用nZVI耦合反硝化系统研究nZVI增强反硝化污泥对有毒化合物降解和系统性能的影响。nZVI 耦合反硝化系统对 2,6-DCP 的影响以及出水 NO ₂ ^-和 NO ₃ ^-的浓度表现出更好的抵抗力低于 2.0 毫克/升。同时，nZVI的加入使反硝化系统在15天内快速适应2,6-DCP的毒性环境，2,6-DCP的降解效率达到99.9%。在 2,6-DCP 环境中 nZVI 与反硝化污泥耦合后释放的 SMP 减少，可以改善出水水质。核磁共振波谱表明，nZVI的加入会改变反硝化污泥中EPS的结构。经过90天的运行，反硝化污泥中的优势菌群发生了很大的变化。此外，塔乌拉是在反硝化系统中降解 2,6-DCP 的主要细菌。NZVI存在下硝酸盐还原、氮呼吸、硝酸盐呼吸和亚硝酸盐呼吸功能菌比例的增加进一步揭示了反硝化作用增强的机制。