The contamination of persistent organic pollutants (POPs), including dichlorodiphenyltrichloroethane (DDT), hexachlorocyclohexane (HCH), and polycyclic aromatic hydrocarbon (PAH), is the most studied environmental issue. In 2020, a total of sixty soil samples collected from ten locations in Guiyang were analyzed to assess the presence of four DDTs and HCHs and sixteen PAHs. The concentrations of total DDTs, total HCHs and Σ₁₆PAHs in the soil were between 0.26 and 12.76, 0.23 and 51.80 μg/kg, and 10.02 and 1708.86 μg/kg, respectively. The mean and median concentrations of total DDTs, total HCHs and Σ₁₆PAHs in the soil were 1.04 and 0.26 μg/kg, 4.32 and 0.23 μg/kg, 139.14 and 98.98 μg/kg, respectively. p,p′-DDT, p,p′-DDD and γ-HCH the dominant organochloride pollutants in the soil, while 4-ring PAHs were the dominant PAHs, occupying 41.1–53.6% of the total PAHs in the soil. The highest levels of PAHs in the soil were observed in areas of Guiyang with relatively larger population densities and more developed heave industries. Various diagnostic tools were used to identify the potential sources of the POPs in the soil. The data indicated that DDTs and HCHs were from past and recent common inputs and that mixtures of several combustion activities (biomass, coal and petroleum combustion, diesel, gasoline, and vehicular emissions) were the major sources of PAHs in the Guiyang soil. The results provide information for the assessment of the extent of POP pollution in the Guiyang soil and can help authorities establish environmental protection regulations and soil remediation techniques.