Steviol glycosides are fully deglycosylated to steviol in the presence of bacterial populations that were isolated from different soil samples. Heating (20 min at 80 °C) or boiling (10 min at 100 °C) of soils had little effect on the steviol formation. It is suggested that bacteria that survived with highly resistant spores are responsible for the deglycosylation of steviol glycosides. A bio-organic preparation method for steviol was developed which had a total yield of 90%. Beside deglycosylation, other reactions also occur. The steviol formed can be degraded. Under anaerobic conditions, rebaudioside A was not hydrolyzed while stevioside was degraded to steviol via rubusoside. Moreover, after an extended incubation (4 weeks) and repetitive sub-cultivation, a bacterial community was selected that converted steviol glycosides to a new and unknown ketone, given the nickname Monicanone. It appeared to be the steviol nucleus without the A-ring that underwent a Walden inversion at its original C-10. A second and related unknown compound could be isolated from an impure preparation of Monicanone by chromatographic separation and purification; this compound was a reduced form of Monicanone and named Monicanol. Steviol glycosides that were incubated with a UASB effluent of an industrial wastewater treatment system – supplemented or not – with sludge of a lab scale nitrification or denitrification unit – were completely degraded via steviol and Monicanone.

在从不同土壤样品中分离出的细菌种群存在的情况下，甜菊糖苷被完全去糖基化为甜菊醇。加热（80°C 下 20 分钟）或沸腾（100°C 下 10 分钟）对甜菊醇的形成几乎没有影响。有人认为，在具有高度抗性孢子的情况下存活下来的细菌负责甜菊糖苷的去糖基化。开发了一种甜菊醇的生物有机制备方法，总收率为90%。除了去糖基化之外，还会发生其他反应。形成的甜菊醇可以降解。在厌氧条件下，莱鲍迪甙 A 不被水解，而甜菊甙通过红枣甙。此外，经过延长的孵育（4 周）和重复的继代培养后，选择了一个细菌群落，将甜菊糖苷转化为一种新的未知酮，绰号为莫尼卡酮。它似乎是没有 A 环的甜菊醇核在其原始 C-10 处经历了瓦尔登湖倒置。通过色谱分离和纯化，可以从不纯的莫尼卡酮制剂中分离出第二种和相关的未知化合物；该化合物是莫尼卡酮的还原形式，命名为莫尼卡醇。甜菊糖苷与工业废水处理系统的 UASB 流出物一起孵育 - 补充或不补充 - 与实验室规模的硝化或反硝化装置的污泥 -通过甜菊醇和莫尼酮完全降解。