Objective

The aim of present study was to determine the effect of interaction among different production variables on esterase production from Serratia sp. EST-4.

Method

The optimum and combined effect of independent parameters were determined using Plackett-Burman and Central Composite Design (CCD) (Design Expert 11).

Results

The effect of six parameters-cotton seed oil, peptone and maltose concentrations, pH, temperature and inoculum volume for production of esterase was determined by Plackett-Burman design. Four factors i.e. inoculum size, pH, peptone concentration and cotton seed oil concentration had significant effect on yield of esterase as suggested by the Pareto chart. Under optimized conditions, (inoculum size (1.0%, v/v), pH (8.0), peptone concentration (1.5%, w/v) and cotton seed oil concentration (4.0%, v/v)), the maximum esterase production was 9.77 U/ml. Analysis of variance (ANOVA) of the Central Composite Design (CCD)-based experiment exhibited the model F-value of 44.24 and P-value of <0.0001 which implies that model was significant and adequate to represent the system.

Conclusion

Inoculum size 1.0%, v/v, pH 8.0, concentration of peptone 1.5%, w/v and concentration of cotton seed oil 4.0%, v/v had positive effect on esterase production and provided 1.52-fold increase in esterase production than that obtained by optimizing one factor at one time.

中文翻译：

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通过响应面法优化培养条件以生产沙雷氏菌胞外酯酶。EST-4

客观的

本研究的目的是确定不同生产变量之间的相互作用对沙雷氏菌酯酶生产的影响。EST-4。

方法

使用 Plackett-Burman 和中央复合设计 (CCD) (Design Expert 11) 确定独立参数的最佳和组合效果。

结果

Plackett-Burman 设计确定了六个参数——棉籽油、蛋白胨和麦芽糖浓度、pH、温度和接种量对酯酶产生的影响。如帕累托图所示，四个因素，即接种物大小、pH、蛋白胨浓度和棉籽油浓度对酯酶的产量有显着影响。在优化条件下（接种量（1.0%，v/v）、pH（8.0）、蛋白胨浓度（1.5%，w/v）和棉籽油浓度（4.0%，v/v）），酯酶产量最大为 9.77 U/ml。基于中央复合设计 (CCD) 的实验的方差分析 (ANOVA) 显示模型 F 值为 44.24，P 值 <0.0001，这意味着该模型是显着的并且足以代表系统。

结论

接种量 1.0%，v/v，pH 8.0，蛋白胨浓度 1.5%，w/v 和棉籽油浓度 4.0%，v/v 对酯酶产量有积极影响，酯酶产量比后者增加 1.52 倍通过一次优化一个因素获得。