The evaluation about the relative distribution of cyanobacterial biomass between the sediment and water column would be indispensable to understand if benthic cyanobacteria are important to cyanobacterial biomass in the water column. A separation method for the rapid quantification of benthic cyanobacteria in Lake Chaohu was developed by density-gradient centrifugation. A 2⁴ full factorial design and response surface methodology was employed to optimize the extraction protocol. Under the optimal operating parameters including 29% Percoll solution, 30 min centrifugation time, 7200 r/min centrifugation speed, and a 1:10 ratio between the volume of sediment and Percoll solution, the recovery rate of cyanobacteria in sediment was 96.73%. Temporal and spatial variations in cyanobacterial biomass in water and sediment were investigated monthly throughout a whole year. In general, cyanobacterial biomass per square centimeter in the water column showed high spatial-temporal changes, tending to increase in February and reaching a peak in April at some sites due to the growth of Dolichospermum. The second peak arrived in July and September and was caused by the rapid growth of Microcystis. Concurrently, cyanobacteria biomass per unit area in sediment showed a clear temporal change pattern, increasing from October and reaching a peak level in February at all the sampling sites. The average ratio of cyanobacterial biomass in water to that in sediment was lowest in January at 1.48 and increased to the highest level in July at 318.61. Although Microcystis and Dolichospermum were dominant species in the water column, only Microcystis was observed in the Percoll solution extraction from sediment. Microscopic observation revealed that a very small fraction of Microcystis cells could survive in sediment, and most of the cells decomposed when the water temperature increased after June. Therefore, the contribution of the recruitment of cyanobacteria could be negligible in Lake Chaohu.

对沉积物和水体之间蓝藻生物量的相对分布的评估对于了解底栖蓝藻是否对水体中的蓝藻生物量很重要是必不可少的。通过密度梯度离心开发了一种用于快速定量巢湖底栖蓝藻的分离方法。一个 2 ⁴采用全因子设计和响应面方法来优化提取方案。在29% Percoll溶液、30 min离心时间、7200 r/min离心速度、底泥体积与Percoll溶液体积比为1:10的最佳操作参数下，底泥中蓝藻的回收率为96.73%。全年每月对水和沉积物中蓝藻生物量的时空变化进行调查。总体而言，水体中每平方厘米蓝藻生物量表现出较大的时空变化，2月份趋于增加，4月份由于白粉菌的生长而在部分地点达到高峰。第二个高峰在 7 月和 9 月到来，是由于微囊藻。同时，沉积物中单位面积蓝藻生物量显示出明显的时间变化模式，从 10 月开始增加，并在所有采样点在 2 月达到峰值。水中蓝藻生物量与沉积物中蓝藻生物量的平均比值在 1 月份最低，为 1.48，在 7 月份上升至最高水平，为 318.61。虽然微囊藻和白粉菌是水体中的优势种，但在从沉积物中提取的 Percoll 溶液中仅观察到微囊藻。显微观察表明，微囊藻中的一小部分细胞可以在沉积物中存活，6月以后水温升高，大部分细胞分解。因此，巢湖中蓝藻补充的贡献可以忽略不计。