Structural changes of two patient-derived glioblastoma cell lines after Zika virus infection were investigated using scanning transmission electron tomography on high-pressure-frozen, freeze-substituted samples. In Zika-virus-infected cells, Golgi structures were barely visible under an electron microscope, and viral factories appeared. The cytosol outside of the viral factories resembled the cytosol of uninfected cells. The viral factories contained largely deranged endoplasmic reticulum (ER), filled with many so-called replication organelles consisting of a luminal vesicle surrounded by the ER membrane. Viral capsids were observed in the vicinity of the replication organelles (cell line #12537 GB) or in ER cisternae at large distance from the replication organelles (cell line #15747 GB). Near the replication organelles, we observed many about 100-nm-long filaments that may represent viral ribonucleoprotein complexes (RNPs), which consist of the RNA genome and N protein oligomers. In addition, we compared Zika-virus-infected cells with cells infected with a phlebovirus (sandfly fever Turkey virus). Zika virions are formed in the ER, whereas phlebovirus virions are assembled in the Golgi apparatus. Our findings will help to understand the replication cycle in the virus factories and the building of the replication organelles in glioblastoma cells.

在高压冷冻、冷冻替代样品上使用扫描透射电子断层扫描研究了寨卡病毒感染后两种患者来源的胶质母细胞瘤细胞系的结构变化。在寨卡病毒感染的细胞中，高尔基体结构在电子显微镜下几乎看不到，并且出现了病毒工厂。病毒工厂外的胞质溶胶类似于未感染细胞的胞质溶胶。病毒工厂包含大部分紊乱的内质网 (ER)，充满了许多所谓的复制细胞器，这些细胞器由被 ER 膜包围的腔囊泡组成。在复制细胞器附近（细胞系#12537 GB）或在距离复制细胞器很远的内质网池（细胞系#15747 GB）中观察到病毒衣壳。在复制细胞器附近，我们观察到许多大约 100 纳米长的细丝，它们可能代表病毒核糖核蛋白复合物 (RNP)，它由 RNA 基因组和 N 蛋白寡聚体组成。此外，我们将感染寨卡病毒的细胞与感染了白蛉病毒（白蛉热土耳其病毒）的细胞进行了比较。寨卡病毒粒子在 ER 中形成，而静脉病毒病毒粒子在高尔基体中组装。我们的研究结果将有助于了解病毒工厂中的复制周期以及胶质母细胞瘤细胞中复制细胞器的构建。而静脉病毒病毒粒子在高尔基体中组装。我们的研究结果将有助于了解病毒工厂中的复制周期以及胶质母细胞瘤细胞中复制细胞器的构建。而静脉病毒病毒粒子在高尔基体中组装。我们的研究结果将有助于了解病毒工厂中的复制周期以及胶质母细胞瘤细胞中复制细胞器的构建。