Glycoproteins play vital roles in living organisms and often serve as biomarkers for some disease. However, due to the low content of glycoprotein in biological fluids, selective detection of glycoproteins is still a challenging issue that needs to be addressed. In this study, molecularly imprinted colloidal array with multi-boronic acid sites for glycoprotein detection under physiological pH was proposed. Monodispersed glycoprotein imprinted particles (SiO₂@PEI/MIPs) was first prepared based on surface imprinting strategy using horseradish peroxidase (HRP) as template, and polyethyleneimine (PEI) was used to increase the number of boronic acid groups. The binding experiment indicated that the SiO₂@PEI/MIPs hold satisfactory adsorption capacity (1.41 μmol/g), rapid adsorption rate (40 min) and preferable selectivity toward HRP. Then the SiO₂@PEI/MIPs was assembled into close-packed colloidal array to construct a label free optical sensor (denoted as GICA). Benefiting from the high ordered photonic crystal structure, binding of HRP onto the GICA could be directly readout from the changes in structure color and diffracted wavelength. The structure color of the GICA changed from bright blue to yellow with the diffraction wavelength red shifted 59 nm when the HRP concentration increased from 2.5 to 15 μmol/L. Importantly, the GICA was capable of detecting HRP from human serum samples. All those results indicated the potential of the GICA for naked-eye detection of glycoprotein.

糖蛋白在生物体中起着至关重要的作用，并且经常作为某些疾病的生物标志物。然而，由于生物体液中糖蛋白含量低，糖蛋白的选择性检测仍然是一个需要解决的具有挑战性的问题。在这项研究中，提出了用于在生理 pH 下检测糖蛋白的具有多硼酸位点的分子印迹胶体阵列。单分散糖蛋白印迹颗粒（SiO ₂ @PEI/MIPs）首先以辣根过氧化物酶（HRP）为模板，基于表面印迹策略制备，聚乙烯亚胺（PEI）用于增加硼酸基团的数量。结合实验表明，SiO ₂@PEI/MIPs 具有令人满意的吸附容量（1.41 μmol/g）、快速的吸附速率（40 分钟）和对 HRP 的优选选择性。然后将 SiO ₂ @PEI/MIP 组装成密堆积的胶体阵列以构建无标记光学传感器（表示为 GICA）。受益于高有序光子晶体结构，HRP 与 GICA 的结合可以直接从结构颜色和衍射波长的变化中读出。当HRP浓度从2.5增至15 μmol/L时，GICA的结构颜色由亮蓝色变为黄色，衍射波长红移59 nm。重要的是，GICA 能够检测人血清样本中的 HRP。所有这些结果都表明 GICA 具有肉眼检测糖蛋白的潜力。