Objective

Long non-coding RNA maternally expressed gene 3 (lnc-MEG3) directly targets microRNA-21 (miR-21) to regulate the vascular microenvironment, and is closely implicated in the pathology of acute ischemic stroke (AIS). However, no research regarding the interaction of lnc-MEG3 and miR-21 in AIS patients has been conducted, to the best of our knowledge. Therefore, we performed this study to evaluate the correlation of lnc-MEG3 with miR-21, and to explore their clinical role for AIS management.

Methods

A total of 170 AIS patients and 100 controls with at least two high-risk factors for stroke were enrolled. The expression of lnc-MEG3 and miR-21 in peripheral blood mononuclear cells was detected by reverse transcription-quantitative polymerase chain reaction.

Results

Lnc-MEG3 expression was increased in AIS patients and could differentiate AIS patients from controls using receiver operating characteristic (ROC) curve analysis with area under the curve (AUC) of 0.874 and a 95% confidence interval (CI) of 0.833–0.914. Lnc-MEG3 expression was positively correlated with tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-17A and the National Institutes of Health Stroke Scale (NIHSS) score, and its high expression was also correlated with elevated accumulating recurrence rate in AIS patients. In addition, lnc-MEG3 expression was negatively correlated with miR-21 expression in AIS patients. Regarding miR-21, it was reduced in AIS patients and could differentiate AIS patients from controls with AUC of 0.889 (95% CI: 0.850–0.927). Also, miR-21 expression was negatively correlated with TNF-α, IL-17A, NIHSS score and accumulating recurrence rate in AIS patients.

Conclusion

Lnc-MEG3 is negatively correlated with miR-21, and both factors are related to disease risk, inflammatory cytokines, disease severity and recurrence risk of AIS.

中文翻译：

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长链非编码RNA母源表达基因3与microRNA-21的关系及其与急性缺血性卒中风险、疾病严重程度和复发风险的相关性

客观的

长链非编码 RNA 母体表达基因 3 (lnc-MEG3) 直接靶向 microRNA-21 (miR-21) 以调节血管微环境，与急性缺血性卒中 (AIS) 的病理学密切相关。然而，据我们所知，尚未进行有关 lnc-MEG3 和 miR-21 在 AIS 患者中相互作用的研究。因此，我们进行了这项研究以评估 lnc-MEG3 与 miR-21 的相关性，并探索它们在 AIS 管理中的临床作用。

方法

共有 170 名 AIS 患者和 100 名具有至少两种卒中高危因素的对照者入组。逆转录-定量聚合酶链反应检测外周血单个核细胞中lnc-MEG3和miR-21的表达。

结果

Lnc-MEG3 在 AIS 患者中的表达增加，并且可以使用受试者工作特征 (ROC) 曲线分析将 AIS 患者与对照组区分开来，曲线下面积 (AUC) 为 0.874，95% 置信区间 (CI) 为 0.833-0.914。Lnc-MEG3 的表达与肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-17A 和美国国立卫生研究院卒中量表（NIHSS）评分呈正相关，其高表达也与累积升高有关。 AIS 患者的复发率。此外，AIS 患者中 lnc-MEG3 的表达与 miR-21 的表达呈负相关。关于 miR-21，它在 AIS 患者中减少，并且可以将 AIS 患者与对照组区分开来，AUC 为 0.889（95% CI：0.850-0.927）。此外，miR-21 表达与 TNF-α、IL-17A、

结论

Lnc-MEG3与miR-21呈负相关，两者均与AIS的疾病风险、炎性细胞因子、疾病严重程度和复发风险相关。