Sulfur dioxide (SO₂) and its derivatives have long been considered as hazardous environmental pollutants but commonly used as food additives in safe dose range. They also could be produced from biological metabolism process of sulfur-containing amino acids. However, their physiological roles remain extremely obscure mainly due to lack of efficient tools for monitoring and imaging strategy establishment. Furthermore, most of current studies of this aspect focus on novel probe design or just imaging them rather than on the ins and outs. Therefore, there is a high significance of establishing highly sensitive detection strategy for monitoring SO₂ derivatives in living systems, food and environment. Herein, we design a fluorescent probe MS-Bindol for sensitively detecting SO₂ derivatives with a low detection limit (0.2 nM). We have established an imaging strategy for investigation of SO₂ derivatives metabolism in living cells and zebrafish, providing visualize evidences and verified that SO₂ derivatives could be synthetized from thiosulfate and glutathione(GSH) and be hardly consumed by using sulfite oxidase inhibitors (ferricyanide or arsenite). Moreover, the probe also exhibits excellent practicability in food as well as environmental samples. Our studies may help biologist for better understanding SO₂ derivatives metabolism and deeply explore their physiological roles in biological systems.

二氧化硫（SO ₂）及其衍生物长期以来一直被认为是有害的环境污染物，但通常用作安全剂量范围内的食品添加剂。它们也可以从含硫氨基酸的生物代谢过程中产生。然而，它们的生理作用仍然非常模糊，主要是由于缺乏有效的监测和成像策略建立工具。此外，目前这方面的大多数研究都集中在新颖的探针设计或仅对它们进行成像而不是在细节上。因此，建立高灵敏度的检测策略对监测生命系统、食品和环境中的SO _{2衍生物具有重要意义。在此，我们设计了一种用于灵敏检测 SO 2}的荧光探针 MS-Bindol具有低检测限 (0.2 nM) 的衍生物。我们建立了用于研究活细胞和斑马鱼中 SO ₂衍生物代谢的成像策略，提供了可视化证据并验证了 SO ₂衍生物可以由硫代硫酸盐和谷胱甘肽（GSH）合成，并且使用亚硫酸盐氧化酶抑制剂（铁氰化物或亚砷酸盐）。此外，该探针在食品和环境样品中也表现出优异的实用性。我们的研究可能有助于生物学家更好地了解 SO ₂衍生物的代谢，并深入探索它们在生物系统中的生理作用。