A Phaseolus vulgaris leukoagglutinin (PHA−L) impedimetric biosensor was developed for the selective detection of cancer-associated N-glycans presenting increased β1→6 GlcNAc branching. The increase in the biosensor‘s impedance after sample incubation was indicative of lectin recognition and complex formation between PHA−L and N-glycans with increased β1→6 GlcNAc branching, present in serum glycoproteins. Bovine thyroglobulin was used as a model glycoprotein and a linear correlation was found between glycoprotein concentration and analytical signal from 0.05 to 2.0 mg ml⁻¹. The biosensor was validated by analyzing serum samples from patients with diverse types of carcinomas.

中文翻译：

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菜豆白细胞凝集素生物传感器作为检测β1→6分支增加的癌症相关N-聚糖的选择性装置

开发了一种普通菜豆白细胞凝集素 (PHA-L) 阻抗生物传感器，用于选择性检测呈现增加的 β1→6 GlcNAc 分支的癌症相关N-聚糖。样品孵育后生物传感器阻抗的增加表明凝集素识别和 PHA-L 和N-聚糖之间的复合物形成，β1→6 GlcNAc 分支增加，存在于血清糖蛋白中。牛甲状腺球蛋白被用作模型糖蛋白并且发现糖蛋白浓度与0.05至2.0mg ml ^{-1 的}分析信号之间存在线性相关性。该生物传感器通过分析来自不同类型癌症患者的血清样本进行了验证。