Microalgal production conducted outdoors using low-cost cultivation media is important for the consolidation of this bioprocess on a large scale. In this context, the use of a chemically defined medium with reduced costs can not only provide nutritional security but also contribute to an increase in the concentration of biomolecules without a loss of microalgal biomass productivity. Thus, this study aimed to evaluate the biomass production and biomolecule concentrations of Spirulina sp. LEB 18 and Synechococcus nidulans LEB 115 in outdoor cultivation using media containing reduced nutrients. Algal performance was assessed in open raceway bioreactors, using three different culture media, namely, BG-11, standard Zarrouk, and a modified Zarrouk [reduction in the sources of carbon (83%), phosphorus (94%), nitrogen (40%), and magnesium (63%) concerning the standard Zarrouk medium]. Cultivation of Spirulina sp. LEB 18 in the modified Zarrouk medium promoted the shortest generation time (2.10 days), with the concentration of carbohydrate produced (37.1%) being 346% higher than that produced using the standard Zarrouk medium (8.3%). Similarly, compared with the Zarrouk medium, the modified Zarrouk medium promoted higher biomass productivity of S. nidulans LEB 115 (0.19 g L⁻¹ d⁻¹) along with a 160% increase in the concentration of carbohydrates (21.6%) produced by this strain. Thus, for the examined microalgal strains, the use of the modified Zarrouk medium enhanced the potential carbohydrate production and maintained the protein concentration above 39%, thereby indicating that this medium would be a promising candidate for the cost-effective large-scale production of microalgal biomass.

使用低成本培养基在户外进行微藻生产对于大规模整合该生物过程非常重要。在这种情况下，使用化学成分确定的培养基不仅可以提供营养安全，而且有助于增加生物分子的浓度，而不会损失微藻生物质生产力。因此，本研究旨在评估螺旋藻的生物质产量和生物分子浓度。LEB 18 和聚球聚球藻LEB 115 在户外栽培中使用营养成分减少的培养基。在开放式水道生物反应器中评估藻类性能，使用三种不同的培养基，即 BG-11、标准 Zarrouk 和改良的 Zarrouk [减少碳源 (83%)、磷 (94%)、氮 (40%) ) 和镁 (63%) 关于标准 Zarrouk 培养基]。螺旋藻的培养。改良 Zarrouk 培养基中的 LEB 18 促进了最短的世代时间（2.10 天），产生的碳水化合物浓度（37.1%）比使用标准 Zarrouk 培养基产生的浓度（8.3%）高 346%。同样，与 Zarrouk 培养基相比，改良的 Zarrouk 培养基促进了S 的更高生物量生产力。nidulans LEB 115 (0.19 g L^-1 d ^-1 ) 以及由该菌株产生的碳水化合物浓度增加 160% (21.6%)。因此，对于所检测的微藻菌株，使用改良的 Zarrouk 培养基提高了潜在的碳水化合物产量，并将蛋白质浓度保持在 39% 以上，从而表明该培养基将成为具有成本效益的大规模微藻生产的有希望的候选者。生物质。