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Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway
Antioxidants ( IF 7 ) Pub Date : 2021-09-12 , DOI: 10.3390/antiox10091445
Pei-Fang Hsieh , Cheng-Chia Yu , Pei-Ming Chu , Pei-Ling Hsieh

Impaired wound healing often occurs in patients with diabetes and causes great inconvenience to them. Aside from the presence of prolonged inflammation, the accumulation of oxidative stress is also implicated in the delayed wound healing. In the present study, we tested the effect of verbascoside, a caffeoyl phenylethanoid glycoside, on the improvement of cell viability and wound healing capacity of gingival epithelial cells under high glucose condition. We showed that verbascoside attenuated the high glucose-induced cytotoxicity and impaired healing, which may be associated with the downregulation of oxidative stress. Our results demonstrated that verbascoside increased the activity of the antioxidant enzyme SOD and reduced the oxidative stress indicator, 8-OHdG, as well as apoptosis. Moreover, verbascoside upregulated the PGC1-α and NRF1 expression and promoted mitochondrial biogenesis, which was mediated by suppression of PKC/HMGB1/RAGE/NFκB signaling. Likewise, we showed the inhibitory effect of verbascoside on oxidative stress was via repression of PKC/HMGB1/RAGE/NFκB activation. Also, our data suggested that the PKC-mediated oxidative stress may lead to the elevated production of inflammatory cytokines, IL-6 and IL-1β. Collectively, we demonstrated that verbascoside may be beneficial to ameliorate impaired oral wound healing for diabetic patients.

中文翻译:

Verbascoside 通过 PKC/HMGB1/RAGE/NFκB 通路保护牙龈细胞免受高糖诱导的氧化应激

糖尿病患者经常会出现伤口愈合障碍,给他们带来很大的不便。除了存在长期炎症外,氧化应激的积累也与伤口愈合延迟有关。在本研究中,我们测试了毛蕊花苷(一种咖啡酰苯乙醇苷)在高糖条件下对提高牙龈上皮细胞的细胞活力和伤口愈合能力的影响。我们发现毛蕊花苷减弱了高葡萄糖诱导的细胞毒性和愈合受损,这可能与氧化应激的下调有关。我们的结果表明,毛蕊花苷增加了抗氧化酶 SOD 的活性,并减少了氧化应激指标 8-OHdG 以及细胞凋亡。而且,毛蕊花苷上调 PGC1-α 和 NRF1 表达并促进线粒体生物发生,这是通过抑制 PKC/HMGB1/RAGE/NFκB 信号传导介导的。同样,我们表明毛蕊花苷对氧化应激的抑制作用是通过抑制 PKC/HMGB1/RAGE/NFκB 激活。此外,我们的数据表明,PKC 介导的氧化应激可能导致炎性细胞因子、IL-6 和 IL-1β 的产生增加。总的来说,我们证明了毛蕊花苷可能有益于改善糖尿病患者口腔伤口愈合受损。我们的数据表明,PKC 介导的氧化应激可能导致炎性细胞因子、IL-6 和 IL-1β 的产生增加。总的来说,我们证明了毛蕊花苷可能有益于改善糖尿病患者口腔伤口愈合受损。我们的数据表明,PKC 介导的氧化应激可能导致炎性细胞因子、IL-6 和 IL-1β 的产生增加。总的来说,我们证明了毛蕊花苷可能有益于改善糖尿病患者口腔伤口愈合受损。
更新日期:2021-09-12
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