Different extraction methods (organic solvent and water extraction with the assistance of heat or ultrasound) were investigated for their effects on the phytochemical profile and antioxidant activity of Clitoria ternatea flowers. For solvent extraction (30%, 50%, 80%, or 100% methanol, ethanol, or acetone), 50% ethanol was considered to be the best solvent for the extraction with an extract yield of 57.3%, a total anthocyanin content of 5.1 mg of cyanidin 3-glucoside equivalent/g of dry weight of extract, and a total phenolic content of 59.4 mg of gallic acid equivalent/g of dry weight of extract. Similar results were obtained for the best water extract at 50 °C for 1 h. A larger number of phytochemicals were identified using LC-MS analysis in the solvent extract (28 compounds). Kaempferol hexosyl-rhamnosyl-rhamnoside was the major compound detected in both the solvent and the water extract. Both extracts showed equally potent antioxidant activity in chemical [IC₅₀ = 1.24 ± 0.05 (solvent extract) and 1.18 ± 0.07 (water extract) mg/mL in the 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay] and cellular (75–80% inhibition) antioxidant assays.

中文翻译：

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有机溶剂和水提取对 Clitoria ternatea 花的植物化学特征和抗氧化活性的影响

研究了不同的提取方法（在热或超声波的帮助下有机溶剂和水提取）对Clitoria ternatea的植物化学特征和抗氧化活性的影响花卉。对于溶剂提取（30%、50%、80% 或 100% 甲醇、乙醇或丙酮），50% 乙醇被认为是提取的最佳溶剂，提取率为 57.3%，总花青素含量为5.1mg花青素3-葡萄糖苷当量/g提取物干重，总酚含量为59.4mg没食子酸当量/g提取物干重。最佳水提取物在 50 °C 下 1 小时获得了类似的结果。使用 LC-MS 分析在溶剂提取物（28 种化合物）中鉴定了大量植物化学物质。Kaempferol hexosyl-rhamnosyl-rhamnoside 是在溶剂和水提取物中检测到的主要化合物。两种提取物在化学 [IC ₅₀ = 1.24 ± 0.05（溶剂提取物）和 1.18 ± 0.07（水提取物）mg/mL，在 2,2-diphenyl-1-picryl-hydrazyl-hydrate 测定] 和细胞（75–80% 抑制）抗氧化测定中。