Lactate is considered to be a signaling molecule that induces mitochondrial adaptation and muscle hypertrophy. The purpose of this study was to examine whether lactate administration attenuates denervation-induced loss of mitochondrial content and muscle mass. Eight-week-old male Institute of Cancer Research mice underwent unilateral sciatic nerve transection surgery. The contralateral hindlimb served as a sham-operated control. From the day of surgery, mice were injected intraperitoneally with PBS or sodium lactate (equivalent to 1 g·kg⁻¹ body weight) once daily for 9 days. After 10 days of denervation, gastrocnemius muscle weight decreased to a similar extent in both the PBS- and lactate-injected groups. Denervation significantly decreased mitochondrial enzyme activity, protein content, and MCT4 protein content in the gastrocnemius muscle. However, lactate administration did not have any significant effects. The current observations suggest that daily lactate administration for 9 days does not affect denervation-induced loss of mitochondrial content and muscle mass.

中文翻译：

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乳酸给药不影响去神经诱导的小鼠线粒体含量和肌肉质量损失

乳酸被认为是诱导线粒体适应和肌肉肥大的信号分子。本研究的目的是检查乳酸给药是否会减轻去神经支配引起的线粒体含量和肌肉质量的损失。八周大的癌症研究所雄性小鼠接受了单侧坐骨神经横断手术。对侧后肢作为假手术对照。从手术当天开始，给小鼠腹腔注射 PBS 或乳酸钠（相当于 1 g·kg ^-1体重）每天一次，持续 9 天。在去神经支配 10 天后，在 PBS 和乳酸注射组中，腓肠肌重量下降到相似程度。去神经显着降低了腓肠肌中的线粒体酶活性、蛋白质含量和 MCT4 蛋白质含量。然而，乳酸给药没有任何显着效果。目前的观察结果表明，每天服用乳酸 9 天不会影响去神经支配引起的线粒体含量和肌肉质量的损失。