Abstract

In this work, in order to explore a new Algerian medicinal plant used in traditional medicine, the essential oil of the leaves of Cachrys sicula L. (Apiaceae) collected from Algeria, obtained by hydrodistillation, was analyzed by GC/MS. Thirty-two compounds were identified accounting for 98.6% of the total oil, which is characterized by a high content of monoterpene hydrocarbons (74.8%). The main constituents of the essential oil were β-pinene (17.9%), sabinene (17.8%), myrcene (12%), and α-pinene (11.4%). In vitro antioxidant activity of the essential oil was assayed by three methods, namely ABTS^•+, metal chelating, and DPPH^• assays. The antioxidant activity of the oil was higher in the ABTS^•+ method. Anticholinesterase activity was screened by the Ellman method against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) which are the chief enzymes of Alzheimer’s disease. The results showed mild acetylcholinesterase and good butyrylcholinesterase inhibitory effects.

摘要

在这项工作中，为了探索一种用于传统医学的新阿尔及利亚药用植物，采用 GC/MS 分析了从阿尔及利亚采集的Cachrys sicula L. (伞形科)叶子的精油，通过加氢蒸馏获得。鉴定出 32 种化合物，占总油量的 98.6%，其特点是单萜烃含量高（74.8%）。精油的主要成分是β-蒎烯（17.9%）、桧烯（17.8%）、月桂烯（12%）和α-蒎烯（11.4%）。精油的体外抗氧化活性通过三种方法测定，即ABTS ^•+、金属螯合和DPPH ^•测定。该油的抗氧化活性在 ABTS ^{•+中更高}方法。抗胆碱酯酶活性采用Ellman 法对阿尔茨海默病的主要酶乙酰胆碱酯酶(AChE) 和丁酰胆碱酯酶(BChE) 进行筛选。结果显示出温和的乙酰胆碱酯酶和良好的丁酰胆碱酯酶抑制作用。