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Pseudomonas putida KT2440 endures temporary oxygen limitations
Biotechnology and Bioengineering ( IF 3.8 ) Pub Date : 2021-09-10 , DOI: 10.1002/bit.27938
Philipp Demling 1 , Andreas Ankenbauer 2 , Bianca Klein 3 , Stephan Noack 3 , Till Tiso 1 , Ralf Takors 2 , Lars M Blank 1
Affiliation  

The obligate aerobic nature of Pseudomonas putida, one of the most prominent whole-cell biocatalysts emerging for industrial bioprocesses, questions its ability to be cultivated in large-scale bioreactors, which exhibit zones of low dissolved oxygen tension. P. putida KT2440 was repeatedly subjected to temporary oxygen limitations in scale-down approaches to assess the effect on growth and an exemplary production of rhamnolipids. At those conditions, the growth and production of P. putida KT2440 were decelerated compared to well-aerated reference cultivations, but remarkably, final biomass and rhamnolipid titers were similar. The robust growth behavior was confirmed across different cultivation systems, media compositions, and laboratories, even when P. putida KT2440 was repeatedly exposed to dual carbon and oxygen starvation. Quantification of the nucleotides ATP, ADP, and AMP revealed a decrease of intracellular ATP concentrations with increasing duration of oxygen starvation, which can, however, be restored when re-supplied with oxygen. Only small changes in the proteome were detected when cells encountered oscillations in dissolved oxygen tensions. Concluding, P. putida KT2440 appears to be able to cope with repeated oxygen limitations as they occur in large-scale bioreactors, affirming its outstanding suitability as a whole-cell biocatalyst for industrial-scale bioprocesses.

中文翻译:

恶臭假单胞菌 KT2440 经受暂时的氧气限制

恶臭假单胞菌是工业生物过程中最突出的全细胞生物催化剂之一,其专性需氧性质质疑其在具有低溶解氧张力区域的大型生物反应器中培养的能力。P. putida KT2440 在按比例缩小的方法中反复受到暂时的氧气限制,以评估对生长的影响和鼠李糖脂的示例性生产。在这些条件下,恶臭假单胞菌KT2440的生长和生产与通气良好的参考培养相比有所减慢,但值得注意的是,最终生物量和鼠李糖脂滴度相似。在不同的培养系统、培养基成分和实验室中证实了强劲的生长行为,即使在P. putida KT2440 反复暴露于碳和氧双重饥饿。核苷酸 ATP、ADP 和 AMP 的定量显示细胞内 ATP 浓度随着缺氧持续时间的增加而降低,然而,当重新供应氧气时可以恢复。当细胞遇到溶解氧张力的波动时,仅检测到蛋白质组的微小变化。最后,恶臭假单胞菌KT2440 似乎能够应对在大型生物反应器中发生的反复氧气限制,证实了其作为全细胞生物催化剂在工业规模生物过程中的出色适用性。
更新日期:2021-11-10
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