当前位置: X-MOL 学术Arch. Insect Biochem. Physiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A role of peptidoglycan recognition protein in mediating insecticide detoxification in Glyphodes pyloalis
Archives of Insect Biochemistry and Physiology ( IF 2.2 ) Pub Date : 2021-09-09 , DOI: 10.1002/arch.21842
De-Lei Jiang 1 , Jian-Hao Ding 1 , Zhi-Xiang Liu 1 , Zuo-Ming Shao 1 , Xin-Hao Liang 1 , Jun Wang 1, 2 , Fu-An Wu 1, 2 , Sheng Sheng 1, 2
Affiliation  

Glyphodes pyloalis Walker has become one of the most significant mulberry pests, and it has caused serious economic losses in major mulberry growing regions in China. Peptidoglycan recognition proteins (PGRPs) are responsible for initiating and regulating immune signalling pathways in insects. However, their roles responding to chemical pesticides is still less known. This study aimed to investigate the possible detoxication function of GpPGRP-S2 and GpPGRP-S3 in G. pyloalis in response to chlorfenapyr and phoxim. The chlorfenapyr and phoxim treatment significantly induced the expression level of GpPGRP-S3 at 48 h. In addition, the expression levels of GpPGRP-S2 and GpPGRP-S3 in the chlorfenapyr/phoxim treatment group were significantly higher in midgut than those in the control group at 48 h. The results of the survival experiment showed that silencing either GpPGRP-S2 or GpPGRP-S3 would not influence the survival rate of G. pyloalis which treated with phoxim, however, silencing GpPGRP-S2 or GpPGRP-S3 would cause G. pyloalis to be more easily killed by chlorfenapyr. The expression of carboxylesterase GpCXE1 was significantly induced by chlorfenapyr/phoxim treatment, while it was suppressed once silenced GpPGRP-S2 followed with chlorfenapyr treatment or silenced GpPGRP-S3 followed with phoxim treatment. These results might suggest that under the chlorfenapyr/phoxim treatment condition, the connection between GpPGRPs and detoxification genes in insect was induced to maintain physiological homeostasis; and these results may further enrich the mechanisms of insects challenged by insecticides.

中文翻译:

肽聚糖识别蛋白在介导 Glyphodes pyloalis 杀虫剂解毒中的作用

Glyphodes pyloalis Walker 已成为最重要的桑树害虫之一,给我国主要桑树产区造成了严重的经济损失。肽聚糖识别蛋白 (PGRP) 负责启动和调节昆虫的免疫信号通路。然而,它们对化学杀虫剂的作用仍然鲜为人知。本研究旨在研究GpPGRP-S2GpPGRP-S3G.pyloalis中对氯苯吡菌胺和辛硫磷的可能解毒作用。氯芬那定和辛硫磷处理在 48 小时显着诱导GpPGRP-S3的表达水平。此外,GpPGRP-S2GpPGRP-S3的表达水平48 h 时,氯芬那定/辛硫磷治疗组中肠中的显着高于对照组。存活实验结果表明,沉默GpPGRP-S2GpPGRP-S3不会影响辛硫磷处理的G.pyloalis存活率,而沉默GpPGRP-S2GpPGRP-S3会导致G.pyloalis更多容易被氯芬那定杀死。羧酸酯酶GpCXE1的表达受到氯苯吡菌胺/辛硫磷处理的显着诱导,而一旦沉默GpPGRP-S2后进行氯苯吡菌胺处理或沉默GpPGRP-S3,它就会被抑制其次是辛硫磷处理。这些结果可能表明,在毒死蜱/辛硫磷处理条件下,诱导了GpPGRPs与昆虫解毒基因之间的联系,以维持生理稳态;这些结果可能进一步丰富了昆虫受到杀虫剂挑战的机制。
更新日期:2021-10-12
down
wechat
bug